Importin 7 may be dispensable for human immunodeficiency virus type 1 and simian immunodeficiency virus infection of primary macrophages

Steven P. Zielske, Mario Stevenson

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

In an in vitro assay employing reconstituted nuclei, importin 7 (IPO7) has been implicated in nuclear translocation of human immunodeficiency virus type 1 (HIV-1) cDNA. Using RNA interference technology, we inhibited expression of IPO7 by 80 to 95% in primary macrophages and in HeLa cells and monitored their ability to support HIV-1 and simian immunodeficiency virus (SIV) cDNA synthesis, nuclear translocation, and infection efficiency. Marked IPO7 deficiency did not alter the rate or extent of HIV-1 or SIV cDNA synthesis or nuclear translocation. The infection efficiency of HIV-1 was similarly unaltered. Therefore, in natural, nondividing targets of HIV-1, IPO7 may be dispensable for infection.

Original languageEnglish
Pages (from-to)11541-11546
Number of pages6
JournalJournal of Virology
Volume79
Issue number17
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

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importins
Karyopherins
Simian immunodeficiency virus
Simian Immunodeficiency Virus
Virus Diseases
Human immunodeficiency virus 1
HIV-1
macrophages
Macrophages
infection
Complementary DNA
Infection
synthesis
RNA Interference
RNA interference
HeLa Cells
Technology
assays

ASJC Scopus subject areas

  • Immunology

Cite this

Importin 7 may be dispensable for human immunodeficiency virus type 1 and simian immunodeficiency virus infection of primary macrophages. / Zielske, Steven P.; Stevenson, Mario.

In: Journal of Virology, Vol. 79, No. 17, 01.09.2005, p. 11541-11546.

Research output: Contribution to journalArticle

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