Implications of altered inotropic effects of phenylephrine in pressure-overloaded cat ventricular muscle

Marion S. Gaide, Jay R. Wiggins, William S. Fitterman, John S. Cameron, Robert J. Myerburg, Arthur L. Bassett

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The positive inotropic action of phenylephrine in cardiac muscle is mediated by α- and β-adrenoceptors. Data suggest the responsiveness of myocardium to inotropic agents is altered in cardiac disease. We evaluated the actions of phenylephrine on isometric contraction and K+-induced contracture in isolated cat right ventricular muscle from normal hearts and hearts with partial pulmonary artery ligation-induced pressure overload of 5-11 days in duration. Peak contractile force (P0) and rate of force development (dP/dt) were lower (p ≤ 0.005) in pressure-overloaded (0.59 ± 0.2 g/mm2 and 4.6 ± 1.7 g/s/mm2, respectively) than in normal (1.33 ± 0.2 g/mm2 and 10.5 ± 1.6 g/s/mm2, respectively) muscle. Phenylephrine (10-6, 5 x 10-6, and 10-5 M) significantly (P ≤ 0.01) increased P0 and dP/dt in normal but not in pressure-overloaded muscle. Phenylephrine (5 x 10-6 M) reduced peak K+-induced contracture force (P(c)) similarly in normal (-30 ± 8%) and pressure-overloaded (-36 ± 11%) muscles. β-Adrenergic blockade (nadolol, 10-4 M) reduced, but did not abolish, the 'relaxant' action of the drug on P(c) in both muscle groups. The lack of a positive inotropic effect of phenylephrine in pressure-overloaded muscle suggests a derangement in both α- and β-adrenoceptor function in this model of cardiac disease.

Original languageEnglish (US)
Pages (from-to)238-243
Number of pages6
JournalJournal of Cardiovascular Pharmacology
Volume6
Issue number2
DOIs
StatePublished - Jan 1 1984

Keywords

  • Isolated ventricular muscle
  • Isometric contraction
  • K-induced contracture
  • Phenylephrine
  • Pressure-overloaded muscle

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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