Implication of mast cell degranulation in ischemic preconditioning-induced prevention of cerebral injury

Ashish K. Rehni, Pradeep Bhateja, Nirmal Singh, Amteshwar S. Jaggi

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The present study has been designed to pharmacologically investigate the role of mast cell degranulation in ischemic preconditioning-induced reversal of global ischemia- and reperfusion-induced cerebral injury in mice. Bilateral carotid artery occlusion of 17 min followed by reperfusion for 24 h was employed in present study to produce ischemia- and reperfusion-induced cerebral injury in mice. Cerebral infarct size was measured using triphenyltetrazolium chloride staining. Memory was evaluated using Morris water-maze test. Rota-rod test was employed to assess motor incoordination. Bilateral carotid artery occlusion followed by reperfusion produced cerebral infarction and impaired memory and motor coordination. Three preceding episodes of bilateral carotid artery occlusion for 1 min and reperfusion of 1 min (ischemic preconditioning) prevented markedly ischemia-reperfusion-induced cerebral injury measured in terms of infarct size, loss of memory and motor coordination. Sodium cromoglycate (10 mg/kg, i.p.), a mast cell stabilizer attenuated the neuroprotective effect of ischemic preconditioning. It is concluded that neuroprotective effect of ischemic preconditioning may be due to the degranulation of mast cells.

Original languageEnglish (US)
Pages (from-to)179-188
Number of pages10
JournalFundamental and Clinical Pharmacology
Volume22
Issue number2
DOIs
StatePublished - Apr 2008
Externally publishedYes

Keywords

  • Cerebral ischemia
  • Ischemic preconditioning
  • Mast cells
  • Sodium cromoglycate

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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