Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts

Nicolas Christoforou, Behzad N. Oskouei, Paul Esteso, Christine M. Hill, Jeffrey M. Zimmet, Weining Bian, Nenad Bursac, Kam W. Leong, Joshua Hare, John D. Gearhart

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Stem cell transplantation holds great promise for the treatment of myocardial infarction injury. We recently described the embryonic stemcell-derived cardiac progenitor cells (CPCs) capable of differentiating into cardiomyocytes, vascular endothelium, and smooth muscle. In this study, we hypothesized that transplanted CPCs will preserve function of the infarcted heart by participating in both muscle replacement and neovascularization. Differentiated CPCs formed functional electromechanical junctions with cardiomyocytes in vitro and conducted action potentials over cm-scale distances. When transplanted into infarctedmouse hearts, CPCs engrafted long-term in the infarct zone and surroundingmyocardium without causing teratomas or arrhythmias. The grafted cells differentiated into cross-striated cardiomyocytes forming gap junctions with the host cells, while also contributing to neovascularization. Serial echocardiography and pressure-volume catheterization demonstrated attenuated ventricular dilatation and preserved left ventricular fractional shortening, systolic and diastolic function. Our results demonstrate that CPCs can engraft, differentiate, and preserve the functional output of the infarcted heart.

Original languageEnglish
Article numbere11536
JournalPLoS One
Volume5
Issue number7
DOIs
StatePublished - Aug 13 2010

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embryonic stem cells
Stem cells
preserves
stem cells
Stem Cells
heart
Cardiac Myocytes
Muscle
mice
Echocardiography
angiogenesis
Gap Junctions
Teratoma
Vascular Endothelium
Stem Cell Transplantation
cell transplantation
gap junctions
Vascular Smooth Muscle
Catheterization
infarction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Christoforou, N., Oskouei, B. N., Esteso, P., Hill, C. M., Zimmet, J. M., Bian, W., ... Gearhart, J. D. (2010). Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts. PLoS One, 5(7), [e11536]. https://doi.org/10.1371/journal.pone.0011536

Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts. / Christoforou, Nicolas; Oskouei, Behzad N.; Esteso, Paul; Hill, Christine M.; Zimmet, Jeffrey M.; Bian, Weining; Bursac, Nenad; Leong, Kam W.; Hare, Joshua; Gearhart, John D.

In: PLoS One, Vol. 5, No. 7, e11536, 13.08.2010.

Research output: Contribution to journalArticle

Christoforou, N, Oskouei, BN, Esteso, P, Hill, CM, Zimmet, JM, Bian, W, Bursac, N, Leong, KW, Hare, J & Gearhart, JD 2010, 'Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts', PLoS One, vol. 5, no. 7, e11536. https://doi.org/10.1371/journal.pone.0011536
Christoforou, Nicolas ; Oskouei, Behzad N. ; Esteso, Paul ; Hill, Christine M. ; Zimmet, Jeffrey M. ; Bian, Weining ; Bursac, Nenad ; Leong, Kam W. ; Hare, Joshua ; Gearhart, John D. / Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts. In: PLoS One. 2010 ; Vol. 5, No. 7.
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