Impairment of reovirus mRNA 'cap' methylation in interferon-treated mouse L929 cells

Ronald C. Desrosiers, Peter Lengyel

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Reovirus mRNAs synthesized in vitro by the virionassociated enzyme have a 5′ 'cap 1' structure (m7G(5′)ppp(5′)GmpCp ...). However, about one third to one half of the reovirus mRNAs formed in mouse L929 cells have a 5′ 'cap 2' structure (m7G(5′)ppp(5′)GmpCmp ...) and the rest have a 5′ 'cap 1' structure. The finding that virus mRNA 'cap' methylation is impaired in extracts of interferon-treated cells prompted us to study the effect of interferon on virus mRNA 'cap' methylation in vivo. Using labeling with [3H]-guanosine and dual labeling with [3H]methionine and [14C]uridine we compared the 5′ structures of reovirus mRNAs accumulating between 5 and 11 h after infection in: L929 cells treated with 390 to 2600 U/ml of a partially purified mouse interferon preparation and untreated L929 cells. The treatment resulted in a 70 to 98% decrease in the 24 h virus yield and in a 50 to 55% decrease in the label accumulated in virus mRNAs. The 'capping' of virus mRNAs and the methylation of their 5′ terminal and adjacent G residues were not diminished in interferon-treated cells. However, the percent of 'cap 2' termini was 36 to 47% lower in virus mRNAs from interferon-treated cells than in virus mRNAs from control cells. The interferon treatment did not result in the appearance of additional methylated nucleotides in the virus mRNAs.

Original languageEnglish (US)
Pages (from-to)471-480
Number of pages10
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume562
Issue number3
DOIs
StatePublished - May 24 1979
Externally publishedYes

Keywords

  • 'Cap' structure
  • (Mouse L929 cell, Reovirus)
  • Interferon
  • mRNA methylation

ASJC Scopus subject areas

  • Medicine(all)

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