Impact of transfusion of mediastinal shed blood on serum levels of cardiac enzymes

Dao Nguyen, Brian M. Gilfix, France Dennis, David Blank, David A. Latter, Patrick L. Ergina, Jean E. Morin, Benoit De Varennes

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background. Infusion of shed mediastinal blood using an autotransfusion system is a widely applied technique of blood conservation in cardiac surgery. Serial determinations of serum creatine kinase (CK), its MB isoenzyme (CK-MB), and lactate hydrogenase (LDH) levels have been used to monitor perioperative myocardial injury. We investigated the impact of postoperative autotransfused blood infusion on serum levels of these enzymes. Methods. We performed a retrospective analysis of postoperative serum CK, CK-MB, and LDH levels of 300 patients who had elective uncomplicated aortocoronary bypass grafting. Shed mediastinal blood samples from 26 patients were analyzed for CK, CK-MB (enzymatic activity and mass), and LDH levels before infusion. Results. High postoperative serum levels of CK and LDH were observed after infusion of autotransfused blood. Shed mediastinal blood contained extremely high levels of these enzymes, particularly from patients who had internal mammary artery dissection. There was a strong correlation (r = 0.96) between measured CK-MB enzyme activities and those calculated from the CK-MB mass units. Conclusions. Infusion of autotransfused blood containing high concentrations of CK and LDH results in elevated serum levels of these enzymes. Hemolysis, frequently present in shed blood, does not interfere with the routine biochemical assays for CK and CK-MB enzyme activities. Caution should be taken when postoperative cardiac enzyme levels are used to determine myocardial injury after aortocoronary bypass grafting if autotransfusion is used as a method of blood conservation.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalAnnals of Thoracic Surgery
Volume62
Issue number1
DOIs
StatePublished - Jul 1 1996
Externally publishedYes

Fingerprint

MB Form Creatine Kinase
Creatine Kinase
Enzymes
Serum
Autologous Blood Transfusions
Coronary Artery Bypass
Hydrogenase
Mammary Arteries
Wounds and Injuries
Hemolysis
Isoenzymes
Thoracic Surgery
Dissection
Lactic Acid

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Nguyen, D., Gilfix, B. M., Dennis, F., Blank, D., Latter, D. A., Ergina, P. L., ... De Varennes, B. (1996). Impact of transfusion of mediastinal shed blood on serum levels of cardiac enzymes. Annals of Thoracic Surgery, 62(1), 109-114. https://doi.org/10.1016/0003-4975(96)00222-6

Impact of transfusion of mediastinal shed blood on serum levels of cardiac enzymes. / Nguyen, Dao; Gilfix, Brian M.; Dennis, France; Blank, David; Latter, David A.; Ergina, Patrick L.; Morin, Jean E.; De Varennes, Benoit.

In: Annals of Thoracic Surgery, Vol. 62, No. 1, 01.07.1996, p. 109-114.

Research output: Contribution to journalArticle

Nguyen, D, Gilfix, BM, Dennis, F, Blank, D, Latter, DA, Ergina, PL, Morin, JE & De Varennes, B 1996, 'Impact of transfusion of mediastinal shed blood on serum levels of cardiac enzymes', Annals of Thoracic Surgery, vol. 62, no. 1, pp. 109-114. https://doi.org/10.1016/0003-4975(96)00222-6
Nguyen, Dao ; Gilfix, Brian M. ; Dennis, France ; Blank, David ; Latter, David A. ; Ergina, Patrick L. ; Morin, Jean E. ; De Varennes, Benoit. / Impact of transfusion of mediastinal shed blood on serum levels of cardiac enzymes. In: Annals of Thoracic Surgery. 1996 ; Vol. 62, No. 1. pp. 109-114.
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