Impact of pancreatic cold preservation on rat islet recovery and function

Antonello Pileggi, Melina M. Ribeiro, Anthony Richard Hogan, Ruth Molano, Lorenzo Cobianchi, Hirohito Ichii, Jennifer Embury, Luca A Inverardi, Alessia Fornoni, Camillo Ricordi, Ricardo Pastori

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

BACKGROUND.: Islet transplantation success depends on the number and quality of islets transplanted. This study aimed at exploring the molecular mechanisms associated with cold pancreas preservation and their impact on islet cell survival and function. METHODS.: Rat pancreata were stored in cold University of Wisconsin preservation solution for short (3 hr; control) or long (18 hr) cold ischemia times (CIT). RESULTS.: Pancreata exposed to long CIT yielded lower islet numbers and showed reduced cellular viability; isolated islets displayed higher levels of phosphorylated stress-activated protein kinase (c-jun N-terminal Kinase and Mitogen-Activated Protein Kinase-p38), and chemokine (C-C) ligand-3, and lower levels of vascular endothelial growth factor, interleukins (IL)-9 and IL-10. Islets obtained from long-CIT pancreata were functionally impaired after transplantation. Differential proteomic expression in pancreatic tissue after CIT included increased eukaryotic translation elongation factor-1-α-1 (apoptosis related) and reduced Clade-B (serine protease inhibitor). CONCLUSIONS.: Our study indicates that cold ischemia stimulates inflammatory pathways (chemokine (c-c)ligand-3, phosphorylation of c-jun N-terminal Kinase and mitogen-activated protein kinase-p38, and eukaryotic translation elongation factor-1-α-1) and decreases repair/cytoprotective pathways (IL-10, vascular endothelial growth factor, and Clade-B), all of which may negatively affect the quality and mass of islets obtained from a donor pancreas.

Original languageEnglish
Pages (from-to)1442-1450
Number of pages9
JournalTransplantation
Volume87
Issue number10
DOIs
StatePublished - May 27 2009

Fingerprint

Cold Ischemia
Recovery of Function
Pancreas
Peptide Elongation Factor 1
p38 Mitogen-Activated Protein Kinases
Interleukin-10
Mitogen-Activated Protein Kinase 10
Vascular Endothelial Growth Factor B
Interleukin-9
Ligands
CC Chemokines
Islets of Langerhans Transplantation
Serine Proteinase Inhibitors
JNK Mitogen-Activated Protein Kinases
Heat-Shock Proteins
Islets of Langerhans
Chemokines
Proteomics
Protein Kinases
Vascular Endothelial Growth Factor A

Keywords

  • Athymic nude mice
  • Cold ischemia
  • Islet cell viability
  • Islet transplantation
  • Lewis rats
  • Pancreas preservation
  • Potency

ASJC Scopus subject areas

  • Transplantation

Cite this

Impact of pancreatic cold preservation on rat islet recovery and function. / Pileggi, Antonello; Ribeiro, Melina M.; Hogan, Anthony Richard; Molano, Ruth; Cobianchi, Lorenzo; Ichii, Hirohito; Embury, Jennifer; Inverardi, Luca A; Fornoni, Alessia; Ricordi, Camillo; Pastori, Ricardo.

In: Transplantation, Vol. 87, No. 10, 27.05.2009, p. 1442-1450.

Research output: Contribution to journalArticle

Pileggi, A, Ribeiro, MM, Hogan, AR, Molano, R, Cobianchi, L, Ichii, H, Embury, J, Inverardi, LA, Fornoni, A, Ricordi, C & Pastori, R 2009, 'Impact of pancreatic cold preservation on rat islet recovery and function', Transplantation, vol. 87, no. 10, pp. 1442-1450. https://doi.org/10.1097/TP.0b013e3181a36b1e
Pileggi A, Ribeiro MM, Hogan AR, Molano R, Cobianchi L, Ichii H et al. Impact of pancreatic cold preservation on rat islet recovery and function. Transplantation. 2009 May 27;87(10):1442-1450. https://doi.org/10.1097/TP.0b013e3181a36b1e
Pileggi, Antonello ; Ribeiro, Melina M. ; Hogan, Anthony Richard ; Molano, Ruth ; Cobianchi, Lorenzo ; Ichii, Hirohito ; Embury, Jennifer ; Inverardi, Luca A ; Fornoni, Alessia ; Ricordi, Camillo ; Pastori, Ricardo. / Impact of pancreatic cold preservation on rat islet recovery and function. In: Transplantation. 2009 ; Vol. 87, No. 10. pp. 1442-1450.
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AU - Cobianchi, Lorenzo

AU - Ichii, Hirohito

AU - Embury, Jennifer

AU - Inverardi, Luca A

AU - Fornoni, Alessia

AU - Ricordi, Camillo

AU - Pastori, Ricardo

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N2 - BACKGROUND.: Islet transplantation success depends on the number and quality of islets transplanted. This study aimed at exploring the molecular mechanisms associated with cold pancreas preservation and their impact on islet cell survival and function. METHODS.: Rat pancreata were stored in cold University of Wisconsin preservation solution for short (3 hr; control) or long (18 hr) cold ischemia times (CIT). RESULTS.: Pancreata exposed to long CIT yielded lower islet numbers and showed reduced cellular viability; isolated islets displayed higher levels of phosphorylated stress-activated protein kinase (c-jun N-terminal Kinase and Mitogen-Activated Protein Kinase-p38), and chemokine (C-C) ligand-3, and lower levels of vascular endothelial growth factor, interleukins (IL)-9 and IL-10. Islets obtained from long-CIT pancreata were functionally impaired after transplantation. Differential proteomic expression in pancreatic tissue after CIT included increased eukaryotic translation elongation factor-1-α-1 (apoptosis related) and reduced Clade-B (serine protease inhibitor). CONCLUSIONS.: Our study indicates that cold ischemia stimulates inflammatory pathways (chemokine (c-c)ligand-3, phosphorylation of c-jun N-terminal Kinase and mitogen-activated protein kinase-p38, and eukaryotic translation elongation factor-1-α-1) and decreases repair/cytoprotective pathways (IL-10, vascular endothelial growth factor, and Clade-B), all of which may negatively affect the quality and mass of islets obtained from a donor pancreas.

AB - BACKGROUND.: Islet transplantation success depends on the number and quality of islets transplanted. This study aimed at exploring the molecular mechanisms associated with cold pancreas preservation and their impact on islet cell survival and function. METHODS.: Rat pancreata were stored in cold University of Wisconsin preservation solution for short (3 hr; control) or long (18 hr) cold ischemia times (CIT). RESULTS.: Pancreata exposed to long CIT yielded lower islet numbers and showed reduced cellular viability; isolated islets displayed higher levels of phosphorylated stress-activated protein kinase (c-jun N-terminal Kinase and Mitogen-Activated Protein Kinase-p38), and chemokine (C-C) ligand-3, and lower levels of vascular endothelial growth factor, interleukins (IL)-9 and IL-10. Islets obtained from long-CIT pancreata were functionally impaired after transplantation. Differential proteomic expression in pancreatic tissue after CIT included increased eukaryotic translation elongation factor-1-α-1 (apoptosis related) and reduced Clade-B (serine protease inhibitor). CONCLUSIONS.: Our study indicates that cold ischemia stimulates inflammatory pathways (chemokine (c-c)ligand-3, phosphorylation of c-jun N-terminal Kinase and mitogen-activated protein kinase-p38, and eukaryotic translation elongation factor-1-α-1) and decreases repair/cytoprotective pathways (IL-10, vascular endothelial growth factor, and Clade-B), all of which may negatively affect the quality and mass of islets obtained from a donor pancreas.

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