Impact of hydration media on ex vivo corneal elasticity measurements

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objectives: To determine the effect of hydration media on ex vivo corneal elasticity. Methods: Experiments were conducted on 40 porcine eyes retrieved from an abattoir (10 eyes each for phosphate-buffered saline (PBS), balanced salt solution, Optisol, 15% dextran). The epithelium was removed, and the cornea was excised with an intact scleral rim and placed in 20% dextran overnight to restore its physiological thickness. For each hydration media, corneas were evenly divided into two groups: one with an intact scleral rim and the other without. Corneas were mounted onto a custom chamber and immersed in a hydration medium for elasticity testing. Although in each medium, corneal elasticity measurements were performed for 2 hr: at 5-min intervals for the first 30 min and then 15-min intervals for the remaining 90 min. Elasticity testing was performed using nanoindentation with spherical indenters, and Young modulus was calculated using the Hertz model. Thickness measurements were taken before and after elasticity testing. Results: The percentage change in corneal thickness and elasticity was calculated for each hydration media group. Balanced salt solution, PBS, and Optisol showed an increase in thickness and Young moduli for corneas with and without an intact scleral rim. Fifteen percent dextran exhibited a dehydrating effect on corneal thickness and provided stable maintenance of corneal elasticity for both groups. Conclusions: Hydration media affects the stability of corneal thickness and elasticity measurements over time. Fifteen percent dextran was most effective in maintaining corneal hydration and elasticity, followed by Optisol.

Original languageEnglish (US)
Pages (from-to)281-286
Number of pages6
JournalEye and Contact Lens
Volume41
Issue number5
DOIs
StatePublished - Sep 11 2015

Fingerprint

Elasticity
Dextrans
Cornea
Elastic Modulus
Salts
Phosphates
Corneal Pachymetry
Abattoirs
Swine
Epithelium
Maintenance

Keywords

  • Atomic force microscopy
  • Cornea
  • Hydration
  • Mechanical properties
  • Nanoindentantion

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Impact of hydration media on ex vivo corneal elasticity measurements. / Dias, Janice; Ziebarth, Noel Marysa.

In: Eye and Contact Lens, Vol. 41, No. 5, 11.09.2015, p. 281-286.

Research output: Contribution to journalArticle

@article{522fd9334dca432797a1090e40207ac3,
title = "Impact of hydration media on ex vivo corneal elasticity measurements",
abstract = "Objectives: To determine the effect of hydration media on ex vivo corneal elasticity. Methods: Experiments were conducted on 40 porcine eyes retrieved from an abattoir (10 eyes each for phosphate-buffered saline (PBS), balanced salt solution, Optisol, 15{\%} dextran). The epithelium was removed, and the cornea was excised with an intact scleral rim and placed in 20{\%} dextran overnight to restore its physiological thickness. For each hydration media, corneas were evenly divided into two groups: one with an intact scleral rim and the other without. Corneas were mounted onto a custom chamber and immersed in a hydration medium for elasticity testing. Although in each medium, corneal elasticity measurements were performed for 2 hr: at 5-min intervals for the first 30 min and then 15-min intervals for the remaining 90 min. Elasticity testing was performed using nanoindentation with spherical indenters, and Young modulus was calculated using the Hertz model. Thickness measurements were taken before and after elasticity testing. Results: The percentage change in corneal thickness and elasticity was calculated for each hydration media group. Balanced salt solution, PBS, and Optisol showed an increase in thickness and Young moduli for corneas with and without an intact scleral rim. Fifteen percent dextran exhibited a dehydrating effect on corneal thickness and provided stable maintenance of corneal elasticity for both groups. Conclusions: Hydration media affects the stability of corneal thickness and elasticity measurements over time. Fifteen percent dextran was most effective in maintaining corneal hydration and elasticity, followed by Optisol.",
keywords = "Atomic force microscopy, Cornea, Hydration, Mechanical properties, Nanoindentantion",
author = "Janice Dias and Ziebarth, {Noel Marysa}",
year = "2015",
month = "9",
day = "11",
doi = "10.1097/ICL.0000000000000119",
language = "English (US)",
volume = "41",
pages = "281--286",
journal = "Eye and Contact Lense",
issn = "1542-2321",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Impact of hydration media on ex vivo corneal elasticity measurements

AU - Dias, Janice

AU - Ziebarth, Noel Marysa

PY - 2015/9/11

Y1 - 2015/9/11

N2 - Objectives: To determine the effect of hydration media on ex vivo corneal elasticity. Methods: Experiments were conducted on 40 porcine eyes retrieved from an abattoir (10 eyes each for phosphate-buffered saline (PBS), balanced salt solution, Optisol, 15% dextran). The epithelium was removed, and the cornea was excised with an intact scleral rim and placed in 20% dextran overnight to restore its physiological thickness. For each hydration media, corneas were evenly divided into two groups: one with an intact scleral rim and the other without. Corneas were mounted onto a custom chamber and immersed in a hydration medium for elasticity testing. Although in each medium, corneal elasticity measurements were performed for 2 hr: at 5-min intervals for the first 30 min and then 15-min intervals for the remaining 90 min. Elasticity testing was performed using nanoindentation with spherical indenters, and Young modulus was calculated using the Hertz model. Thickness measurements were taken before and after elasticity testing. Results: The percentage change in corneal thickness and elasticity was calculated for each hydration media group. Balanced salt solution, PBS, and Optisol showed an increase in thickness and Young moduli for corneas with and without an intact scleral rim. Fifteen percent dextran exhibited a dehydrating effect on corneal thickness and provided stable maintenance of corneal elasticity for both groups. Conclusions: Hydration media affects the stability of corneal thickness and elasticity measurements over time. Fifteen percent dextran was most effective in maintaining corneal hydration and elasticity, followed by Optisol.

AB - Objectives: To determine the effect of hydration media on ex vivo corneal elasticity. Methods: Experiments were conducted on 40 porcine eyes retrieved from an abattoir (10 eyes each for phosphate-buffered saline (PBS), balanced salt solution, Optisol, 15% dextran). The epithelium was removed, and the cornea was excised with an intact scleral rim and placed in 20% dextran overnight to restore its physiological thickness. For each hydration media, corneas were evenly divided into two groups: one with an intact scleral rim and the other without. Corneas were mounted onto a custom chamber and immersed in a hydration medium for elasticity testing. Although in each medium, corneal elasticity measurements were performed for 2 hr: at 5-min intervals for the first 30 min and then 15-min intervals for the remaining 90 min. Elasticity testing was performed using nanoindentation with spherical indenters, and Young modulus was calculated using the Hertz model. Thickness measurements were taken before and after elasticity testing. Results: The percentage change in corneal thickness and elasticity was calculated for each hydration media group. Balanced salt solution, PBS, and Optisol showed an increase in thickness and Young moduli for corneas with and without an intact scleral rim. Fifteen percent dextran exhibited a dehydrating effect on corneal thickness and provided stable maintenance of corneal elasticity for both groups. Conclusions: Hydration media affects the stability of corneal thickness and elasticity measurements over time. Fifteen percent dextran was most effective in maintaining corneal hydration and elasticity, followed by Optisol.

KW - Atomic force microscopy

KW - Cornea

KW - Hydration

KW - Mechanical properties

KW - Nanoindentantion

UR - http://www.scopus.com/inward/record.url?scp=84941258891&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941258891&partnerID=8YFLogxK

U2 - 10.1097/ICL.0000000000000119

DO - 10.1097/ICL.0000000000000119

M3 - Article

VL - 41

SP - 281

EP - 286

JO - Eye and Contact Lense

JF - Eye and Contact Lense

SN - 1542-2321

IS - 5

ER -