Impact of chromosomal rearrangement upon DNA methylation patterns in leukemia

Hyang Min Byun, Shahrooz Eshaghian, Dan Douer, Jonathen Trent, Guillermo Garcia-Manero, Ravi Bhatia, Kim Siegmund, Allen S. Yang

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Genomic instability, including genetic mutations and chromosomal rearrangements, can lead to cancer development. Aberrant DNA methylation occurs commonly in cancer cells. The aim of this study is to determine the effects of a specific chromosomal lesion the BCR-ABL translocation t(9:22), in establishing DNA methylation profiles in cancer. Materials and methods We compared DNA methylation of 1,505 selected promoter CpGs in chronic myelogenous leukemia (CML), acute lymphoblastic leukemia (ALL) with and without the Philadelphia chromosome t(9:22), CD34+ hematopoietic stem cells transfected with BCR-ABL, and other tumors without BCR-ABL (acute promyelocytic leukemia (APL) and gastrointestinal stromal tumors (GIST). In this study, the DNA methylation profile of CML was more closely related to APL, another myeloid leukemia, than Ph+ ALL. Although DNA methylation profiles were consistent within a specific tumor type, overall DNA methylation profiles were no influenced by BCR-ABL gene translocation in the cancers and tissues studied. We conclude that DNA methylation profiles may reflect the cell of origin in cancers rather than the chromosomal lesions involved in leukemogenesis.

Original languageEnglish (US)
Pages (from-to)76-85
Number of pages10
JournalOpen Medicine (Poland)
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2017

Keywords

  • 5-azacytidine
  • Acute Lymphoblastic Leukemia
  • Acute Promyelocytic Leukemia
  • Chronic Myelogenous Leukemia
  • DNA methylation
  • Gastrointestinal Stromal Tumor
  • Philadelphia Chromosome

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Byun, H. M., Eshaghian, S., Douer, D., Trent, J., Garcia-Manero, G., Bhatia, R., Siegmund, K., & Yang, A. S. (2017). Impact of chromosomal rearrangement upon DNA methylation patterns in leukemia. Open Medicine (Poland), 12(1), 76-85. https://doi.org/10.1515/med-2017-0014