Immunoreactive endothelin-1 concentrations in follicular fluid of women with and without endometriosis undergoing in vitro fertilization-embryo transfer

M. Abae, M. Glassberg, M. H. Majercik, H. Yoshida, R. Vestal, D. Puett

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Objective: To determine the concentrations of immunoreactive (IR) endothelin-1 in human follicular fluid (FF) and whether IR-endothelin-1 levels are different in women with endometriosis-associated infertility. Design: Follicular fluid and plasma samples, obtained from women with and without endometriosis undergoing IVF-ET, were collected at the time of oocyte aspiration and analyzed for IR-endothelin-1 levels. Setting: Infertility clinic in an academic research environment. Results: Overall, 90% of FF samples and 60% of plasma samples contained IR-endothelin-1 detectable above the threshold of assay sensitivity. Immunoreactive endothelin-1 levels (mean ± SEM) in FF samples from women with and without endometriosis-associated infertility were 74 ± 12 and 37 ± 6 pg/mL, respectively. There was no difference in IR-endothelin-1 levels in FF samples between controlled ovarian hyperstimulation cycles with or without leuprolide acetate. No significant differences were detected in plasma IR-endothelin-1 levels in women with endometriosis-associated infertility when compared with those without. Conclusions: These results demonstrate the presence of IR-endothelin-1 in human FF obtained at the time of oocyte aspiration for IVF-ET and higher levels of IR-endothelin-1 in FF of women with endometriosis-associated infertility.

Original languageEnglish (US)
Pages (from-to)1083-1087
Number of pages5
JournalFertility and sterility
Volume61
Issue number6
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Keywords

  • endometriosis
  • Endothelin
  • follicular fluid
  • IVF-ET

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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