Abstract
Reagents are now available which allow simultaneous assessment of three different fluorescence wavelengths on most commercially available flow cytometers. Such three-color analyses provide more information than single- or dual-color analyses. The present study was undertaken in order to establish age-related differences in lymphocyte subpopulations by simultaneously measuring three surface antigens in newborns, children, and adults. A whole blood method was used to label cells with antibodies conjugated to FITC, PE, and perCP. We found that the percentage of lymphocytes expressing HLA-DR/CD28/CD8, HLA-DR/CD38/CD8, CD95/CD45RO/CD8, CD95/CD45RO/CD4, CD95/CD4, and CD95/CD8 showed relative increases with age. The percentage of lymphocytes expressing CD28/CD8, CD38/CD8, and CD38/CD4 showed relative decreases with age, while the subset HLA-DR/CD38/CD4 did not change. Three-color flow cytometry is a powerful tool to more precisely define lymphocyte subsets than the current two-color methods. We present values using a three-color panel in healthy newborns, children, and adults.
| Original language | English |
|---|---|
| Pages (from-to) | 46-55 |
| Number of pages | 10 |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 84 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Pathology and Forensic Medicine
Cite this
Immunophenotyping of T lymphocytes by three-color flow cytometry in healthy newborns, children, and adults. / McCloskey, Thomas W.; Cavaliere, Terri; Bakshi, Saroj; Harper, Rita; Fagin, James; Kohn, Nina; Pahwa, Savita G.
In: Clinical Immunology and Immunopathology, Vol. 84, No. 1, 01.07.1997, p. 46-55.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunophenotyping of T lymphocytes by three-color flow cytometry in healthy newborns, children, and adults
AU - McCloskey, Thomas W.
AU - Cavaliere, Terri
AU - Bakshi, Saroj
AU - Harper, Rita
AU - Fagin, James
AU - Kohn, Nina
AU - Pahwa, Savita G
PY - 1997/7/1
Y1 - 1997/7/1
N2 - Reagents are now available which allow simultaneous assessment of three different fluorescence wavelengths on most commercially available flow cytometers. Such three-color analyses provide more information than single- or dual-color analyses. The present study was undertaken in order to establish age-related differences in lymphocyte subpopulations by simultaneously measuring three surface antigens in newborns, children, and adults. A whole blood method was used to label cells with antibodies conjugated to FITC, PE, and perCP. We found that the percentage of lymphocytes expressing HLA-DR/CD28/CD8, HLA-DR/CD38/CD8, CD95/CD45RO/CD8, CD95/CD45RO/CD4, CD95/CD4, and CD95/CD8 showed relative increases with age. The percentage of lymphocytes expressing CD28/CD8, CD38/CD8, and CD38/CD4 showed relative decreases with age, while the subset HLA-DR/CD38/CD4 did not change. Three-color flow cytometry is a powerful tool to more precisely define lymphocyte subsets than the current two-color methods. We present values using a three-color panel in healthy newborns, children, and adults.
AB - Reagents are now available which allow simultaneous assessment of three different fluorescence wavelengths on most commercially available flow cytometers. Such three-color analyses provide more information than single- or dual-color analyses. The present study was undertaken in order to establish age-related differences in lymphocyte subpopulations by simultaneously measuring three surface antigens in newborns, children, and adults. A whole blood method was used to label cells with antibodies conjugated to FITC, PE, and perCP. We found that the percentage of lymphocytes expressing HLA-DR/CD28/CD8, HLA-DR/CD38/CD8, CD95/CD45RO/CD8, CD95/CD45RO/CD4, CD95/CD4, and CD95/CD8 showed relative increases with age. The percentage of lymphocytes expressing CD28/CD8, CD38/CD8, and CD38/CD4 showed relative decreases with age, while the subset HLA-DR/CD38/CD4 did not change. Three-color flow cytometry is a powerful tool to more precisely define lymphocyte subsets than the current two-color methods. We present values using a three-color panel in healthy newborns, children, and adults.
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U2 - 10.1006/clin.1997.4370
DO - 10.1006/clin.1997.4370
M3 - Article
C2 - 9191883
AN - SCOPUS:0030740573
VL - 84
SP - 46
EP - 55
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 1
ER -