Immunophenotyping of T lymphocytes by three-color flow cytometry in healthy newborns, children, and adults

Thomas W. McCloskey, Terri Cavaliere, Saroj Bakshi, Rita Harper, James Fagin, Nina Kohn, Savita G Pahwa

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Reagents are now available which allow simultaneous assessment of three different fluorescence wavelengths on most commercially available flow cytometers. Such three-color analyses provide more information than single- or dual-color analyses. The present study was undertaken in order to establish age-related differences in lymphocyte subpopulations by simultaneously measuring three surface antigens in newborns, children, and adults. A whole blood method was used to label cells with antibodies conjugated to FITC, PE, and perCP. We found that the percentage of lymphocytes expressing HLA-DR/CD28/CD8, HLA-DR/CD38/CD8, CD95/CD45RO/CD8, CD95/CD45RO/CD4, CD95/CD4, and CD95/CD8 showed relative increases with age. The percentage of lymphocytes expressing CD28/CD8, CD38/CD8, and CD38/CD4 showed relative decreases with age, while the subset HLA-DR/CD38/CD4 did not change. Three-color flow cytometry is a powerful tool to more precisely define lymphocyte subsets than the current two-color methods. We present values using a three-color panel in healthy newborns, children, and adults.

Original languageEnglish
Pages (from-to)46-55
Number of pages10
JournalClinical Immunology and Immunopathology
Volume84
Issue number1
DOIs
StatePublished - Jul 1 1997
Externally publishedYes

Fingerprint

Immunophenotyping
Flow Cytometry
Color
Newborn Infant
T-Lymphocytes
HLA-DR Antigens
Lymphocyte Subsets
Lymphocytes
Fluorescein-5-isothiocyanate
Surface Antigens
Fluorescence
Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pathology and Forensic Medicine

Cite this

Immunophenotyping of T lymphocytes by three-color flow cytometry in healthy newborns, children, and adults. / McCloskey, Thomas W.; Cavaliere, Terri; Bakshi, Saroj; Harper, Rita; Fagin, James; Kohn, Nina; Pahwa, Savita G.

In: Clinical Immunology and Immunopathology, Vol. 84, No. 1, 01.07.1997, p. 46-55.

Research output: Contribution to journalArticle

McCloskey, Thomas W. ; Cavaliere, Terri ; Bakshi, Saroj ; Harper, Rita ; Fagin, James ; Kohn, Nina ; Pahwa, Savita G. / Immunophenotyping of T lymphocytes by three-color flow cytometry in healthy newborns, children, and adults. In: Clinical Immunology and Immunopathology. 1997 ; Vol. 84, No. 1. pp. 46-55.
@article{27d4ccfa93ef4be2a29d80fbbe670928,
title = "Immunophenotyping of T lymphocytes by three-color flow cytometry in healthy newborns, children, and adults",
abstract = "Reagents are now available which allow simultaneous assessment of three different fluorescence wavelengths on most commercially available flow cytometers. Such three-color analyses provide more information than single- or dual-color analyses. The present study was undertaken in order to establish age-related differences in lymphocyte subpopulations by simultaneously measuring three surface antigens in newborns, children, and adults. A whole blood method was used to label cells with antibodies conjugated to FITC, PE, and perCP. We found that the percentage of lymphocytes expressing HLA-DR/CD28/CD8, HLA-DR/CD38/CD8, CD95/CD45RO/CD8, CD95/CD45RO/CD4, CD95/CD4, and CD95/CD8 showed relative increases with age. The percentage of lymphocytes expressing CD28/CD8, CD38/CD8, and CD38/CD4 showed relative decreases with age, while the subset HLA-DR/CD38/CD4 did not change. Three-color flow cytometry is a powerful tool to more precisely define lymphocyte subsets than the current two-color methods. We present values using a three-color panel in healthy newborns, children, and adults.",
author = "McCloskey, {Thomas W.} and Terri Cavaliere and Saroj Bakshi and Rita Harper and James Fagin and Nina Kohn and Pahwa, {Savita G}",
year = "1997",
month = "7",
day = "1",
doi = "10.1006/clin.1997.4370",
language = "English",
volume = "84",
pages = "46--55",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Immunophenotyping of T lymphocytes by three-color flow cytometry in healthy newborns, children, and adults

AU - McCloskey, Thomas W.

AU - Cavaliere, Terri

AU - Bakshi, Saroj

AU - Harper, Rita

AU - Fagin, James

AU - Kohn, Nina

AU - Pahwa, Savita G

PY - 1997/7/1

Y1 - 1997/7/1

N2 - Reagents are now available which allow simultaneous assessment of three different fluorescence wavelengths on most commercially available flow cytometers. Such three-color analyses provide more information than single- or dual-color analyses. The present study was undertaken in order to establish age-related differences in lymphocyte subpopulations by simultaneously measuring three surface antigens in newborns, children, and adults. A whole blood method was used to label cells with antibodies conjugated to FITC, PE, and perCP. We found that the percentage of lymphocytes expressing HLA-DR/CD28/CD8, HLA-DR/CD38/CD8, CD95/CD45RO/CD8, CD95/CD45RO/CD4, CD95/CD4, and CD95/CD8 showed relative increases with age. The percentage of lymphocytes expressing CD28/CD8, CD38/CD8, and CD38/CD4 showed relative decreases with age, while the subset HLA-DR/CD38/CD4 did not change. Three-color flow cytometry is a powerful tool to more precisely define lymphocyte subsets than the current two-color methods. We present values using a three-color panel in healthy newborns, children, and adults.

AB - Reagents are now available which allow simultaneous assessment of three different fluorescence wavelengths on most commercially available flow cytometers. Such three-color analyses provide more information than single- or dual-color analyses. The present study was undertaken in order to establish age-related differences in lymphocyte subpopulations by simultaneously measuring three surface antigens in newborns, children, and adults. A whole blood method was used to label cells with antibodies conjugated to FITC, PE, and perCP. We found that the percentage of lymphocytes expressing HLA-DR/CD28/CD8, HLA-DR/CD38/CD8, CD95/CD45RO/CD8, CD95/CD45RO/CD4, CD95/CD4, and CD95/CD8 showed relative increases with age. The percentage of lymphocytes expressing CD28/CD8, CD38/CD8, and CD38/CD4 showed relative decreases with age, while the subset HLA-DR/CD38/CD4 did not change. Three-color flow cytometry is a powerful tool to more precisely define lymphocyte subsets than the current two-color methods. We present values using a three-color panel in healthy newborns, children, and adults.

UR - http://www.scopus.com/inward/record.url?scp=0030740573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030740573&partnerID=8YFLogxK

U2 - 10.1006/clin.1997.4370

DO - 10.1006/clin.1997.4370

M3 - Article

C2 - 9191883

AN - SCOPUS:0030740573

VL - 84

SP - 46

EP - 55

JO - Clinical Immunology

JF - Clinical Immunology

SN - 1521-6616

IS - 1

ER -