TY - JOUR
T1 - Immunophenotyping of T lymphocytes by three-color flow cytometry in healthy newborns, children, and adults
AU - McCloskey, Thomas W.
AU - Cavaliere, Terri
AU - Bakshi, Saroj
AU - Harper, Rita
AU - Fagin, James
AU - Kohn, Nina
AU - Pahwa, Savita
N1 - Funding Information:
This research was supported by NIH Grants AI28281 and DA05161. We thank Becton±Dickinson Immunocytometry Systems for providing the McAbs used in this study. We also thank Dr. Martin Lesser from the Department of Biostatistics for helpful advice on data analysis. In addition, we are grateful to Regina Pasieka, Maria Marecki, Corinne Leombruno, and Maria Coronesi for technical assistance.
PY - 1997/7
Y1 - 1997/7
N2 - Reagents are now available which allow simultaneous assessment of three different fluorescence wavelengths on most commercially available flow cytometers. Such three-color analyses provide more information than single- or dual-color analyses. The present study was undertaken in order to establish age-related differences in lymphocyte subpopulations by simultaneously measuring three surface antigens in newborns, children, and adults. A whole blood method was used to label cells with antibodies conjugated to FITC, PE, and perCP. We found that the percentage of lymphocytes expressing HLA-DR/CD28/CD8, HLA-DR/CD38/CD8, CD95/CD45RO/CD8, CD95/CD45RO/CD4, CD95/CD4, and CD95/CD8 showed relative increases with age. The percentage of lymphocytes expressing CD28/CD8, CD38/CD8, and CD38/CD4 showed relative decreases with age, while the subset HLA-DR/CD38/CD4 did not change. Three-color flow cytometry is a powerful tool to more precisely define lymphocyte subsets than the current two-color methods. We present values using a three-color panel in healthy newborns, children, and adults.
AB - Reagents are now available which allow simultaneous assessment of three different fluorescence wavelengths on most commercially available flow cytometers. Such three-color analyses provide more information than single- or dual-color analyses. The present study was undertaken in order to establish age-related differences in lymphocyte subpopulations by simultaneously measuring three surface antigens in newborns, children, and adults. A whole blood method was used to label cells with antibodies conjugated to FITC, PE, and perCP. We found that the percentage of lymphocytes expressing HLA-DR/CD28/CD8, HLA-DR/CD38/CD8, CD95/CD45RO/CD8, CD95/CD45RO/CD4, CD95/CD4, and CD95/CD8 showed relative increases with age. The percentage of lymphocytes expressing CD28/CD8, CD38/CD8, and CD38/CD4 showed relative decreases with age, while the subset HLA-DR/CD38/CD4 did not change. Three-color flow cytometry is a powerful tool to more precisely define lymphocyte subsets than the current two-color methods. We present values using a three-color panel in healthy newborns, children, and adults.
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U2 - 10.1006/clin.1997.4370
DO - 10.1006/clin.1997.4370
M3 - Article
C2 - 9191883
AN - SCOPUS:0030740573
VL - 84
SP - 46
EP - 55
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 1
ER -