Abstract
The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.
| Original language | English |
|---|---|
| Pages (from-to) | 38-47 |
| Number of pages | 10 |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 52 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pathology and Forensic Medicine
Cite this
Immunophenotyping in a multicenter study : The Transfusion Safety Study experience. / Fletcher, Mary Ann; Azen, Stanley P.; Adelsberg, Bernard; Gjerset, George; Hassett, Joseph; Kaplan, Joseph; Niland, Joyce C.; Odom-Maryon, Tamara; Parker, John W.; Stites, Daniel P.; Mosley, James W.
In: Clinical Immunology and Immunopathology, Vol. 52, No. 1, 01.01.1989, p. 38-47.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunophenotyping in a multicenter study
T2 - The Transfusion Safety Study experience
AU - Fletcher, Mary Ann
AU - Azen, Stanley P.
AU - Adelsberg, Bernard
AU - Gjerset, George
AU - Hassett, Joseph
AU - Kaplan, Joseph
AU - Niland, Joyce C.
AU - Odom-Maryon, Tamara
AU - Parker, John W.
AU - Stites, Daniel P.
AU - Mosley, James W.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.
AB - The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.
UR - http://www.scopus.com/inward/record.url?scp=0024322297&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024322297&partnerID=8YFLogxK
U2 - 10.1016/0090-1229(89)90191-8
DO - 10.1016/0090-1229(89)90191-8
M3 - Article
C2 - 2656018
AN - SCOPUS:0024322297
VL - 52
SP - 38
EP - 47
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 1
ER -