Immunophenotyping in a multicenter study: The Transfusion Safety               Study experience

Mary Ann Fletcher; Stanley P. Azen; Bernard Adelsberg; George Gjerset; Joseph Hassett; Joseph Kaplan; Joyce C. Niland; Tamara Odom-Maryon; John W. Parker; Daniel P. Stites; James W. Mosley

doi:10.1016/0090-1229(89)90191-8

Immunophenotyping in a multicenter study: The Transfusion Safety Study experience

Mary Ann Fletcher, Stanley P. Azen, Bernard Adelsberg, George Gjerset, Joseph Hassett, Joseph Kaplan, Joyce C. Niland, Tamara Odom-Maryon, John W. Parker, Daniel P. Stites, James W. Mosley

Medicine

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.

Original language	English (US)
Pages (from-to)	38-47
Number of pages	10
Journal	Clinical Immunology and Immunopathology
Volume	52
Issue number	1
DOIs	https://doi.org/10.1016/0090-1229(89)90191-8
State	Published - Jul 1989

ASJC Scopus subject areas

Immunology and Allergy
Pathology and Forensic Medicine
Immunology

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

Access to Document

10.1016/0090-1229(89)90191-8

Fingerprint Dive into the research topics of 'Immunophenotyping in a multicenter study: The Transfusion Safety Study experience'. Together they form a unique fingerprint.

Cite this

Immunophenotyping in a multicenter study : The Transfusion Safety Study experience. / Fletcher, Mary Ann; Azen, Stanley P.; Adelsberg, Bernard; Gjerset, George; Hassett, Joseph; Kaplan, Joseph; Niland, Joyce C.; Odom-Maryon, Tamara; Parker, John W.; Stites, Daniel P.; Mosley, James W.

In: Clinical Immunology and Immunopathology, Vol. 52, No. 1, 07.1989, p. 38-47.

Research output: Contribution to journal › Article › peer-review

Fletcher, Mary Ann ; Azen, Stanley P. ; Adelsberg, Bernard ; Gjerset, George ; Hassett, Joseph ; Kaplan, Joseph ; Niland, Joyce C. ; Odom-Maryon, Tamara ; Parker, John W. ; Stites, Daniel P. ; Mosley, James W. / Immunophenotyping in a multicenter study : The Transfusion Safety Study experience. In: Clinical Immunology and Immunopathology. 1989 ; Vol. 52, No. 1. pp. 38-47.

@article{9412730afb6e41df893cd154a7683302,

title = "Immunophenotyping in a multicenter study: The Transfusion Safety Study experience",

abstract = "The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.",

author = "Fletcher, {Mary Ann} and Azen, {Stanley P.} and Bernard Adelsberg and George Gjerset and Joseph Hassett and Joseph Kaplan and Niland, {Joyce C.} and Tamara Odom-Maryon and Parker, {John W.} and Stites, {Daniel P.} and Mosley, {James W.}",

note = "Funding Information: {\textquoteright} Supported by contract No. NOI-HB-4-7003 of the National Heart, Lung, and Blood Institute, National Institutes of Health. Presented as part of a symposium entitled “Evaluation of the Immune System during HIV Infection and AIDS: T Cell Subsets,” November 3. 1988. San Francisco, CA. 2 To whom correspondence should be addressed. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1989",

month = jul,

doi = "10.1016/0090-1229(89)90191-8",

language = "English (US)",

volume = "52",

pages = "38--47",

journal = "Clinical Immunology",

issn = "1521-6616",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Immunophenotyping in a multicenter study

T2 - The Transfusion Safety Study experience

AU - Fletcher, Mary Ann

AU - Azen, Stanley P.

AU - Adelsberg, Bernard

AU - Gjerset, George

AU - Hassett, Joseph

AU - Kaplan, Joseph

AU - Niland, Joyce C.

AU - Odom-Maryon, Tamara

AU - Parker, John W.

AU - Stites, Daniel P.

AU - Mosley, James W.

N1 - Funding Information: ’ Supported by contract No. NOI-HB-4-7003 of the National Heart, Lung, and Blood Institute, National Institutes of Health. Presented as part of a symposium entitled “Evaluation of the Immune System during HIV Infection and AIDS: T Cell Subsets,” November 3. 1988. San Francisco, CA. 2 To whom correspondence should be addressed. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1989/7

Y1 - 1989/7

N2 - The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.

AB - The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.

UR - http://www.scopus.com/inward/record.url?scp=0024322297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024322297&partnerID=8YFLogxK

U2 - 10.1016/0090-1229(89)90191-8

DO - 10.1016/0090-1229(89)90191-8

M3 - Article

C2 - 2656018

AN - SCOPUS:0024322297

VL - 52

SP - 38

EP - 47

JO - Clinical Immunology

JF - Clinical Immunology

SN - 1521-6616

IS - 1