Immunophenotyping in a multicenter study: The Transfusion Safety Study experience

Mary Ann Fletcher, Stanley P. Azen, Bernard Adelsberg, George Gjerset, Joseph Hassett, Joseph Kaplan, Joyce C. Niland, Tamara Odom-Maryon, John W. Parker, Daniel P. Stites, James W. Mosley

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The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.

Original languageEnglish (US)
Pages (from-to)38-47
Number of pages10
JournalClinical Immunology and Immunopathology
Issue number1
StatePublished - Jul 1989

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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    Fletcher, M. A., Azen, S. P., Adelsberg, B., Gjerset, G., Hassett, J., Kaplan, J., Niland, J. C., Odom-Maryon, T., Parker, J. W., Stites, D. P., & Mosley, J. W. (1989). Immunophenotyping in a multicenter study: The Transfusion Safety Study experience. Clinical Immunology and Immunopathology, 52(1), 38-47.