Immunophenotyping in a multicenter study: The Transfusion Safety Study experience

Mary Ann Fletcher, Stanley P. Azen, Bernard Adelsberg, George Gjerset, Joseph Hassett, Joseph Kaplan, Joyce C. Niland, Tamara Odom-Maryon, John W. Parker, Daniel P. Stites, James W. Mosley

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.

Original languageEnglish
Pages (from-to)38-47
Number of pages10
JournalClinical Immunology and Immunopathology
Volume52
Issue number1
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

CD8-Positive T-Lymphocytes
Immunophenotyping
Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Multicenter Studies
HIV-1
CD4-Positive T-Lymphocytes
Safety
Quality Control
Monocytes
Flow Cytometry
Color
Monoclonal Antibodies
Infection

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Fletcher, M. A., Azen, S. P., Adelsberg, B., Gjerset, G., Hassett, J., Kaplan, J., ... Mosley, J. W. (1989). Immunophenotyping in a multicenter study: The Transfusion Safety Study experience. Clinical Immunology and Immunopathology, 52(1), 38-47. https://doi.org/10.1016/0090-1229(89)90191-8

Immunophenotyping in a multicenter study : The Transfusion Safety Study experience. / Fletcher, Mary Ann; Azen, Stanley P.; Adelsberg, Bernard; Gjerset, George; Hassett, Joseph; Kaplan, Joseph; Niland, Joyce C.; Odom-Maryon, Tamara; Parker, John W.; Stites, Daniel P.; Mosley, James W.

In: Clinical Immunology and Immunopathology, Vol. 52, No. 1, 01.01.1989, p. 38-47.

Research output: Contribution to journalArticle

Fletcher, MA, Azen, SP, Adelsberg, B, Gjerset, G, Hassett, J, Kaplan, J, Niland, JC, Odom-Maryon, T, Parker, JW, Stites, DP & Mosley, JW 1989, 'Immunophenotyping in a multicenter study: The Transfusion Safety Study experience', Clinical Immunology and Immunopathology, vol. 52, no. 1, pp. 38-47. https://doi.org/10.1016/0090-1229(89)90191-8
Fletcher, Mary Ann ; Azen, Stanley P. ; Adelsberg, Bernard ; Gjerset, George ; Hassett, Joseph ; Kaplan, Joseph ; Niland, Joyce C. ; Odom-Maryon, Tamara ; Parker, John W. ; Stites, Daniel P. ; Mosley, James W. / Immunophenotyping in a multicenter study : The Transfusion Safety Study experience. In: Clinical Immunology and Immunopathology. 1989 ; Vol. 52, No. 1. pp. 38-47.
@article{9412730afb6e41df893cd154a7683302,
title = "Immunophenotyping in a multicenter study: The Transfusion Safety Study experience",
abstract = "The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.",
author = "Fletcher, {Mary Ann} and Azen, {Stanley P.} and Bernard Adelsberg and George Gjerset and Joseph Hassett and Joseph Kaplan and Niland, {Joyce C.} and Tamara Odom-Maryon and Parker, {John W.} and Stites, {Daniel P.} and Mosley, {James W.}",
year = "1989",
month = "1",
day = "1",
doi = "10.1016/0090-1229(89)90191-8",
language = "English",
volume = "52",
pages = "38--47",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Immunophenotyping in a multicenter study

T2 - The Transfusion Safety Study experience

AU - Fletcher, Mary Ann

AU - Azen, Stanley P.

AU - Adelsberg, Bernard

AU - Gjerset, George

AU - Hassett, Joseph

AU - Kaplan, Joseph

AU - Niland, Joyce C.

AU - Odom-Maryon, Tamara

AU - Parker, John W.

AU - Stites, Daniel P.

AU - Mosley, James W.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.

AB - The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation. reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.

UR - http://www.scopus.com/inward/record.url?scp=0024322297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024322297&partnerID=8YFLogxK

U2 - 10.1016/0090-1229(89)90191-8

DO - 10.1016/0090-1229(89)90191-8

M3 - Article

C2 - 2656018

AN - SCOPUS:0024322297

VL - 52

SP - 38

EP - 47

JO - Clinical Immunology

JF - Clinical Immunology

SN - 1521-6616

IS - 1

ER -