Immunophenotypic characterization of acute leukemia by immunocytology

A. J. Fishleder, R. R. Tubbs, R. A. Savage, F. V. Lucas, D. Norris, J. K. Weick, R. Valenzuela

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The potential for specific immunophenotypic characterization of the acute leukemias has been enhanced greatly by the development of monoclonal antibodies. Currently, this immunologic data is obtained most commonly by flow cytometric analysis or cellular cytotoxicity assays. The former is an expensive technic that lacks morphologic evaluation unless cell storing is performed. The latter precludes morphologic assessment by the nature of the assay. The authors have developed an immunostaining procedure utilizing cytospin preparations and immunoperoxidase methods that are relatively inexpensive and allow simultaneous assessment of the immunologic markers and cellular morphology. Although a comparison of flow cytometry and immunocytology revealed quantitative differences for individual cell surface markers, the 'qualitative' immunologic phenotype of the leukemic population was virtually identical by the two technics.

Original languageEnglish (US)
Pages (from-to)611-617
Number of pages7
JournalAmerican journal of clinical pathology
Volume81
Issue number5
DOIs
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Fishleder, A. J., Tubbs, R. R., Savage, R. A., Lucas, F. V., Norris, D., Weick, J. K., & Valenzuela, R. (1984). Immunophenotypic characterization of acute leukemia by immunocytology. American journal of clinical pathology, 81(5), 611-617. https://doi.org/10.1093/ajcp/81.5.611