Immunophenotypic characterization of acute leukemia by immunocytology

A. J. Fishleder, R. R. Tubbs, R. A. Savage, F. V. Lucas, D. Norris, J. K. Weick, R. Valenzuela

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The potential for specific immunophenotypic characterization of the acute leukemias has been enhanced greatly by the development of monoclonal antibodies. Currently, this immunologic data is obtained most commonly by flow cytometric analysis or cellular cytotoxicity assays. The former is an expensive technic that lacks morphologic evaluation unless cell storing is performed. The latter precludes morphologic assessment by the nature of the assay. The authors have developed an immunostaining procedure utilizing cytospin preparations and immunoperoxidase methods that are relatively inexpensive and allow simultaneous assessment of the immunologic markers and cellular morphology. Although a comparison of flow cytometry and immunocytology revealed quantitative differences for individual cell surface markers, the 'qualitative' immunologic phenotype of the leukemic population was virtually identical by the two technics.

Original languageEnglish
Pages (from-to)611-617
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume81
Issue number5
StatePublished - Jun 22 1984
Externally publishedYes

Fingerprint

Leukemia
Surface Antigens
Individuality
Flow Cytometry
Biomarkers
Monoclonal Antibodies
Phenotype
Population

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Fishleder, A. J., Tubbs, R. R., Savage, R. A., Lucas, F. V., Norris, D., Weick, J. K., & Valenzuela, R. (1984). Immunophenotypic characterization of acute leukemia by immunocytology. American Journal of Clinical Pathology, 81(5), 611-617.

Immunophenotypic characterization of acute leukemia by immunocytology. / Fishleder, A. J.; Tubbs, R. R.; Savage, R. A.; Lucas, F. V.; Norris, D.; Weick, J. K.; Valenzuela, R.

In: American Journal of Clinical Pathology, Vol. 81, No. 5, 22.06.1984, p. 611-617.

Research output: Contribution to journalArticle

Fishleder, AJ, Tubbs, RR, Savage, RA, Lucas, FV, Norris, D, Weick, JK & Valenzuela, R 1984, 'Immunophenotypic characterization of acute leukemia by immunocytology', American Journal of Clinical Pathology, vol. 81, no. 5, pp. 611-617.
Fishleder AJ, Tubbs RR, Savage RA, Lucas FV, Norris D, Weick JK et al. Immunophenotypic characterization of acute leukemia by immunocytology. American Journal of Clinical Pathology. 1984 Jun 22;81(5):611-617.
Fishleder, A. J. ; Tubbs, R. R. ; Savage, R. A. ; Lucas, F. V. ; Norris, D. ; Weick, J. K. ; Valenzuela, R. / Immunophenotypic characterization of acute leukemia by immunocytology. In: American Journal of Clinical Pathology. 1984 ; Vol. 81, No. 5. pp. 611-617.
@article{4e775e52486b44c3b291f58b2555c999,
title = "Immunophenotypic characterization of acute leukemia by immunocytology",
abstract = "The potential for specific immunophenotypic characterization of the acute leukemias has been enhanced greatly by the development of monoclonal antibodies. Currently, this immunologic data is obtained most commonly by flow cytometric analysis or cellular cytotoxicity assays. The former is an expensive technic that lacks morphologic evaluation unless cell storing is performed. The latter precludes morphologic assessment by the nature of the assay. The authors have developed an immunostaining procedure utilizing cytospin preparations and immunoperoxidase methods that are relatively inexpensive and allow simultaneous assessment of the immunologic markers and cellular morphology. Although a comparison of flow cytometry and immunocytology revealed quantitative differences for individual cell surface markers, the 'qualitative' immunologic phenotype of the leukemic population was virtually identical by the two technics.",
author = "Fishleder, {A. J.} and Tubbs, {R. R.} and Savage, {R. A.} and Lucas, {F. V.} and D. Norris and Weick, {J. K.} and R. Valenzuela",
year = "1984",
month = "6",
day = "22",
language = "English",
volume = "81",
pages = "611--617",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "5",

}

TY - JOUR

T1 - Immunophenotypic characterization of acute leukemia by immunocytology

AU - Fishleder, A. J.

AU - Tubbs, R. R.

AU - Savage, R. A.

AU - Lucas, F. V.

AU - Norris, D.

AU - Weick, J. K.

AU - Valenzuela, R.

PY - 1984/6/22

Y1 - 1984/6/22

N2 - The potential for specific immunophenotypic characterization of the acute leukemias has been enhanced greatly by the development of monoclonal antibodies. Currently, this immunologic data is obtained most commonly by flow cytometric analysis or cellular cytotoxicity assays. The former is an expensive technic that lacks morphologic evaluation unless cell storing is performed. The latter precludes morphologic assessment by the nature of the assay. The authors have developed an immunostaining procedure utilizing cytospin preparations and immunoperoxidase methods that are relatively inexpensive and allow simultaneous assessment of the immunologic markers and cellular morphology. Although a comparison of flow cytometry and immunocytology revealed quantitative differences for individual cell surface markers, the 'qualitative' immunologic phenotype of the leukemic population was virtually identical by the two technics.

AB - The potential for specific immunophenotypic characterization of the acute leukemias has been enhanced greatly by the development of monoclonal antibodies. Currently, this immunologic data is obtained most commonly by flow cytometric analysis or cellular cytotoxicity assays. The former is an expensive technic that lacks morphologic evaluation unless cell storing is performed. The latter precludes morphologic assessment by the nature of the assay. The authors have developed an immunostaining procedure utilizing cytospin preparations and immunoperoxidase methods that are relatively inexpensive and allow simultaneous assessment of the immunologic markers and cellular morphology. Although a comparison of flow cytometry and immunocytology revealed quantitative differences for individual cell surface markers, the 'qualitative' immunologic phenotype of the leukemic population was virtually identical by the two technics.

UR - http://www.scopus.com/inward/record.url?scp=0021355739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021355739&partnerID=8YFLogxK

M3 - Article

VL - 81

SP - 611

EP - 617

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 5

ER -