Immunoperoxidase techniques, although new, have already led to major advances in our understanding of many skin disorders of infectious, autoimmune, and neoplastic nature. Because of their versatility, sensitivity, and specificity, immunoperoxidase procedures are the best 'special' stains if appropriate antibodies are available. These techniques often obviate the requirement for older, nonspecific histologic stains or for electron microscopy, which is more cumbersome, costly, and time consuming. Although immunoperoxidase staining is simple for any qualified technician to do, there are numerous pitfalls, and correct interpretation of its reactions requires skill and experience. Few authors have addressed problems associated with immunoperoxidase procedures. This paper therefore discusses the principles of the technique, its pitfalls, and steps that should be taken to avoid errors.
ASJC Scopus subject areas
- Pathology and Forensic Medicine