Desarrollo de características inmunopatológicas en el intestino medio del mosquito después de alimentarse de ratones inmunizados con Mucina-1 / Interleucina-12 ADNc de Anopheles gambiae

Translated title of the contribution: Immunopathological features developing in the mosquito midgut after feeding on Anopheles gambiae Mucin-1 / Interleukin-12 cDNA immunized mice

Wilfred Emonyi Injera, Elphantus W. Kabiru, Michael M. Gicheru, John I. Githure, John C Beier

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The mosquito midgut is the organ into which the blood meal passes and in which, within a peritrophic membrane secreted by the epithelium, the blood is retained during digestion and absorption. The mosquito midgut is lined with an actin filled microvilli that are exposed to the harsh environment of the gut lumen such as food particle abrasion, digestive hydrolases and attack by pathogens and parasites that are taken in by the blood meal. These microvilli are protected them these effects by the peritrophic matrix, the glycocalyx and the mucin proteins that line their epithelial surfaces. Immunization of BALB/c mice with AgMUC1/IL-12 cDNA has been shown to kill mosquitoes when fed on these mice. Mucin is one of the proteins produced in the mosquito midgut after a blood meal. The fine structure of the mosquito midgut epithelium interacting with immune factors such as antibodies or immune cells is of special significance for interpreting early events in the interaction between the mosquito midgut lining and the specific immune components present in the blood of AgMUC1/IL-12 cDNA immunized BALB/c mice. Following bright light microscopy, scanning electron and transmission electron microscopic observations of the features seen in mosquito midgut sections from An. gambiae mosquitoes fed on BALB/c mice immunized with AgMUC1/IL-12 cDNA, the most likely immune mechanisms responsible for mosquito killing could be cell mediated, most likely antibody dependent cellular cytotoxicity. Both necrotic and apoptotic processes that could be the cause of mosquito death were seen to take place in the cells lining the midgut epithelium.

Original languageSpanish
Pages (from-to)329-337
Number of pages9
JournalInternational Journal of Morphology
Volume31
Issue number1
DOIs
StatePublished - Mar 1 2013

Fingerprint

Anopheles gambiae
Mucin-1
Interleukin-12
Culicidae
Complementary DNA
Meals
Epithelium
Mucins
Microvilli
Electrons
Glycocalyx
Antibodies
Immunologic Factors
Hydrolases
Actins
Cause of Death
Digestion
Microscopy
Immunization
Parasites

Keywords

  • Activated hemocytes
  • Adherent cells
  • Immunopathological features
  • Midgut epithelium

ASJC Scopus subject areas

  • Anatomy

Cite this

Desarrollo de características inmunopatológicas en el intestino medio del mosquito después de alimentarse de ratones inmunizados con Mucina-1 / Interleucina-12 ADNc de Anopheles gambiae. / Injera, Wilfred Emonyi; Kabiru, Elphantus W.; Gicheru, Michael M.; Githure, John I.; Beier, John C.

In: International Journal of Morphology, Vol. 31, No. 1, 01.03.2013, p. 329-337.

Research output: Contribution to journalArticle

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