Immunomodulatory effects of sensory nerves during respiratory syncytial virus infection in rats

Alexander Auais, Rebecca D Adkins, Galia Napchan, Giovanni Piedimonte

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Respiratory syncytial virus (RSV) infection is associated with exaggerated neurogenic inflammation in the airways. This study sought to determine whether irritation of the mucosal sensory fibers affects the recruitment of lymphocytes and monocytes to RSV-infected airways. Pathogen-free rats were inoculated with RSV or with virus-free medium and were injected 5 days later with capsaicin to stimulate airway sensory nerves. Bronchoalveolar lavage was performed 1, 5, or 10 days after nerve stimulation, and samples were analyzed by differential cell count and flow cytometry. Without nerve stimulation, RSV caused a minimal increase in the number of lymphocytes and monocytes above pathogen-free control levels. After nerve stimulation, numerous lymphocytes, predominantly CD4+ T cells, and monocytes were recruited in the airways of infected rats, whereas no difference was found in pathogen-free controls. RSV induced overexpression of the neurokinin 1 (NK1) receptor for substance P on discrete lymphocyte subpopulations within the bronchial-associated lymphoid tissue (BALT), and treatment with a specific NK1 receptor antagonist abolished the recruitment of both lymphocytes and monocytes to infected airways. Our data suggest that airborne irritants stimulating mucosal sensory fibers during RSV infection exert important immunomodulatory effects by attracting to the infected airways selected lymphocyte subpopulations from the local BALT as well as monocytes.

Original languageEnglish
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume285
Issue number1 29-1
StatePublished - Jul 1 2003

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Respiratory Syncytial Virus Infections
Respiratory Syncytial Viruses
Monocytes
Lymphocyte Subsets
Lymphoid Tissue
Lymphocytes
Neurokinin-1 Receptor Antagonists
Neurogenic Inflammation
Neurokinin-1 Receptors
Irritants
Capsaicin
Lymphocyte Count
Bronchoalveolar Lavage
Substance P
Flow Cytometry
Cell Count
Viruses
T-Lymphocytes

Keywords

  • Airway inflammation
  • Asthma
  • Lymphocytes
  • Monocytes
  • Substance P

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

Cite this

Immunomodulatory effects of sensory nerves during respiratory syncytial virus infection in rats. / Auais, Alexander; Adkins, Rebecca D; Napchan, Galia; Piedimonte, Giovanni.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 285, No. 1 29-1, 01.07.2003.

Research output: Contribution to journalArticle

Auais, Alexander ; Adkins, Rebecca D ; Napchan, Galia ; Piedimonte, Giovanni. / Immunomodulatory effects of sensory nerves during respiratory syncytial virus infection in rats. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2003 ; Vol. 285, No. 1 29-1.
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