Immunological, pharmacological, and electrophysiological detection of T-cell-modulating properties of substances of abuse

Robert M. Donahoe, John J. Madden, Dorothy R. Oleson, Charles B. Nemeroff

Research output: Contribution to journalArticle

Abstract

The chapter describes immunobiological, immunopharmacological, and immuno electrophysiological techniques that are useful for in vitro reconstruction of the in vivo milieu associated with substance abuse. When studying the complexities involved with the in vivo effects of opiates and other substances of abuse such as cocaine and alcohol, it is important to realize that the initial site of interaction of the immune system with such substances and the products they induce within the neuroimmune system is the surface membrane of the immune cell. To simplify studies of the etiology of these interactions, the chapter has taken an in vitro, reductionistic approach aimed at determining the influence of opiates, other substances of abuse, and neuroimmune produces induced by these substances, on membrane-receptor and ion-channel phenomena. To further reduce the complexity of this task while maintaining the relevance of the studies to cell-mediated immune deficits encountered by substance abusers, the chapter targeted T cells for study.

Original languageEnglish (US)
Pages (from-to)410-423
Number of pages14
JournalMethods in Neurosciences
Volume24
Issue numberC
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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