Immunological identification of a high molecular weight protein as a candidate for the product of the Duchenne muscular dystrophy gene

L. Kao; J. Krstenansky; J. Mendell; K. W. Rammohan; E. Gruenstein

doi:10.1073/pnas.85.12.4491

Immunological identification of a high molecular weight protein as a candidate for the product of the Duchenne muscular dystrophy gene

L. Kao, J. Krstenansky, J. Mendell, K. W. Rammohan, E. Gruenstein

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

An oligopeptide was synthesized based on translation of the nucleotide sequence of the putative exon region of clone pERT87-25 from the gene for Duchenne muscular dystrophy. Immunization of rabbits with this oligopeptide induced the formation of antibodies directed against a protein present in human, rat, and rabbit skeletal muscle. This protein, which is missing in the skeletal muscle of two patients with Duchenne muscular dystrophy, has a molecular mass of ~320-420 kDa and is clearly different from the putative Duchenne muscular dystrophy-related protein nebulin. The data suggest that this 320- to 420-kDa protein is produced by the Duchenne muscular dystrophy gene.

Original language	English (US)
Pages (from-to)	4491-4495
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	85
Issue number	12
DOIs	https://doi.org/10.1073/pnas.85.12.4491
State	Published - 1988
Externally published	Yes

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Immunological identification of a high molecular weight protein as a candidate for the product of the Duchenne muscular dystrophy gene. / Kao, L.; Krstenansky, J.; Mendell, J.; Rammohan, K. W.; Gruenstein, E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 12, 1988, p. 4491-4495.

Research output: Contribution to journal › Article › peer-review

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abstract = "An oligopeptide was synthesized based on translation of the nucleotide sequence of the putative exon region of clone pERT87-25 from the gene for Duchenne muscular dystrophy. Immunization of rabbits with this oligopeptide induced the formation of antibodies directed against a protein present in human, rat, and rabbit skeletal muscle. This protein, which is missing in the skeletal muscle of two patients with Duchenne muscular dystrophy, has a molecular mass of ~320-420 kDa and is clearly different from the putative Duchenne muscular dystrophy-related protein nebulin. The data suggest that this 320- to 420-kDa protein is produced by the Duchenne muscular dystrophy gene.",

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AU - Gruenstein, E.

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