Abstract
An oligopeptide was synthesized based on translation of the nucleotide sequence of the putative exon region of clone pERT87-25 from the gene for Duchenne muscular dystrophy. Immunization of rabbits with this oligopeptide induced the formation of antibodies directed against a protein present in human, rat, and rabbit skeletal muscle. This protein, which is missing in the skeletal muscle of two patients with Duchenne muscular dystrophy, has a molecular mass of ~320-420 kDa and is clearly different from the putative Duchenne muscular dystrophy-related protein nebulin. The data suggest that this 320- to 420-kDa protein is produced by the Duchenne muscular dystrophy gene.
| Original language | English |
|---|---|
| Pages (from-to) | 4491-4495 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 85 |
| Issue number | 12 |
| State | Published - Jan 1 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- General
- Genetics
Cite this
Immunological identification of a high molecular weight protein as a candidate for the product of the Duchenne muscular dystrophy gene. / Kao, L.; Krstenansky, J.; Mendell, J.; Rammohan, Kottil W; Gruenstein, E.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 12, 01.01.1988, p. 4491-4495.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunological identification of a high molecular weight protein as a candidate for the product of the Duchenne muscular dystrophy gene
AU - Kao, L.
AU - Krstenansky, J.
AU - Mendell, J.
AU - Rammohan, Kottil W
AU - Gruenstein, E.
PY - 1988/1/1
Y1 - 1988/1/1
N2 - An oligopeptide was synthesized based on translation of the nucleotide sequence of the putative exon region of clone pERT87-25 from the gene for Duchenne muscular dystrophy. Immunization of rabbits with this oligopeptide induced the formation of antibodies directed against a protein present in human, rat, and rabbit skeletal muscle. This protein, which is missing in the skeletal muscle of two patients with Duchenne muscular dystrophy, has a molecular mass of ~320-420 kDa and is clearly different from the putative Duchenne muscular dystrophy-related protein nebulin. The data suggest that this 320- to 420-kDa protein is produced by the Duchenne muscular dystrophy gene.
AB - An oligopeptide was synthesized based on translation of the nucleotide sequence of the putative exon region of clone pERT87-25 from the gene for Duchenne muscular dystrophy. Immunization of rabbits with this oligopeptide induced the formation of antibodies directed against a protein present in human, rat, and rabbit skeletal muscle. This protein, which is missing in the skeletal muscle of two patients with Duchenne muscular dystrophy, has a molecular mass of ~320-420 kDa and is clearly different from the putative Duchenne muscular dystrophy-related protein nebulin. The data suggest that this 320- to 420-kDa protein is produced by the Duchenne muscular dystrophy gene.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023764874&partnerID=8YFLogxK
M3 - Article
C2 - 3288996
AN - SCOPUS:0023764874
VL - 85
SP - 4491
EP - 4495
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 12
ER -