Immunological identification of a high molecular weight protein as a candidate for the product of the Duchenne muscular dystrophy gene

L. Kao, J. Krstenansky, J. Mendell, Kottil W Rammohan, E. Gruenstein

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

An oligopeptide was synthesized based on translation of the nucleotide sequence of the putative exon region of clone pERT87-25 from the gene for Duchenne muscular dystrophy. Immunization of rabbits with this oligopeptide induced the formation of antibodies directed against a protein present in human, rat, and rabbit skeletal muscle. This protein, which is missing in the skeletal muscle of two patients with Duchenne muscular dystrophy, has a molecular mass of ~320-420 kDa and is clearly different from the putative Duchenne muscular dystrophy-related protein nebulin. The data suggest that this 320- to 420-kDa protein is produced by the Duchenne muscular dystrophy gene.

Original languageEnglish
Pages (from-to)4491-4495
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number12
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Duchenne Muscular Dystrophy
Molecular Weight
Oligopeptides
Genes
Skeletal Muscle
Proteins
Rabbits
Antibody Formation
Exons
Immunization
Clone Cells

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Immunological identification of a high molecular weight protein as a candidate for the product of the Duchenne muscular dystrophy gene. / Kao, L.; Krstenansky, J.; Mendell, J.; Rammohan, Kottil W; Gruenstein, E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 12, 01.01.1988, p. 4491-4495.

Research output: Contribution to journalArticle

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