Immunological detection of Zika virus: A summary in the context of general viral diagnostics

Rebecca Peters, Mario Stevenson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Zika virus (ZIKV) captured mass public attention in 2014–16 due to its potentially devastating teratogenic effects in unborn babies. Although recent years show a decline in disease activity as ZIKV settles into endemic New World cycles, the significant challenges that originally complicated its diagnosis remain, and linkages to foetal microcephaly make safe, accurate, and timely detection especially important. The present chapter seeks to define current direct and indirect immunological detection methods for ZIKV in the context of cross-reactive viral relatives, and will consider the utility and difficulties associated with each practice in resource-rich and resource-poor settings. A synthesis of general diagnostic approaches will more specifically cover immunological techniques spanning from serology to antigen-based immunoassay against the backdrop of common luminescent, fluorescent, and colorimetric output signal types. This analysis uses the diagnostic landscape of Zika virus as a ready template for the classification and contextual understanding of many methods within viral diagnostics.

Original languageEnglish (US)
Title of host publicationMethods in Microbiology
PublisherAcademic Press Inc.
Pages275-318
Number of pages44
DOIs
StatePublished - 2020

Publication series

NameMethods in Microbiology
Volume47
ISSN (Print)0580-9517

Keywords

  • Antigen-based detection
  • Colorimetry
  • Cross-reactivity
  • Detection
  • Diagnosis
  • Diagnostic
  • Diagnostics
  • ELISA
  • Enzyme-linked immunosorbent assay
  • Fluorescence
  • Immunoassay
  • Immunological
  • Lateral flow assay
  • LFA
  • Luminescence
  • Plaque reduction neutralization test
  • PRNT
  • Serological
  • Viral diagnostics
  • Zika virus
  • ZIKV

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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  • Cite this

    Peters, R., & Stevenson, M. (2020). Immunological detection of Zika virus: A summary in the context of general viral diagnostics. In Methods in Microbiology (pp. 275-318). (Methods in Microbiology; Vol. 47). Academic Press Inc.. https://doi.org/10.1016/bs.mim.2019.11.009