Immunolocalization of X-arrestin in human cone photoreceptors

H. Sakuma; G. Inana; A. Murakami; T. Higashide; M. J. McLaren

doi:10.1016/0014-5793(96)00163-9

Immunolocalization of X-arrestin in human cone photoreceptors

H. Sakuma, G. Inana, A. Murakami, T. Higashide, M. J. McLaren

Ophthalmology

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

X-arrestin is a recently identified retina-specific gene of unknown function. Affinity-purified anti-peptide antibody to human X-arrestin was prepared, and used in Western blot analysis of human retinal proteins and for immunohistochemistry on human retinal sections. By Western blot analysis, the antibody specifically bound to an ~47 kDa protein, and by indirect immunofluorescence specifically labeled cone photoreceptors with greatest intensity in their outer segments. In single and double label experiments, the localization of X-arrestin immunoreactivity was compared with immunolabeling patterns obtained with antibodies to red/green cone opsin, rhodopsin, and S-antigen. The results showed that X-arrestin is expressed in red-, green- and blue-sensitive cones in the human retina.

Original language	English (US)
Pages (from-to)	105-110
Number of pages	6
Journal	FEBS letters
Volume	382
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(96)00163-9
State	Published - Mar 11 1996

Keywords

Anti-peptide antibody
Arrestin
Human cone photoreceptor
Immunofluorescence
Western blot analysis
X-Arrestin

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Access to Document

10.1016/0014-5793(96)00163-9

Fingerprint Dive into the research topics of 'Immunolocalization of X-arrestin in human cone photoreceptors'. Together they form a unique fingerprint.

Cite this

@article{1efa313d6f2242388f5ba4a0400ed7c4,

title = "Immunolocalization of X-arrestin in human cone photoreceptors",

abstract = "X-arrestin is a recently identified retina-specific gene of unknown function. Affinity-purified anti-peptide antibody to human X-arrestin was prepared, and used in Western blot analysis of human retinal proteins and for immunohistochemistry on human retinal sections. By Western blot analysis, the antibody specifically bound to an ~47 kDa protein, and by indirect immunofluorescence specifically labeled cone photoreceptors with greatest intensity in their outer segments. In single and double label experiments, the localization of X-arrestin immunoreactivity was compared with immunolabeling patterns obtained with antibodies to red/green cone opsin, rhodopsin, and S-antigen. The results showed that X-arrestin is expressed in red-, green- and blue-sensitive cones in the human retina.",

keywords = "Anti-peptide antibody, Arrestin, Human cone photoreceptor, Immunofluorescence, Western blot analysis, X-Arrestin",

author = "H. Sakuma and G. Inana and A. Murakami and T. Higashide and McLaren, {M. J.}",

note = "Funding Information: Acknowledgements: Supported by the Foundation Fighting Blindness, Baltimore, MD, Research to Prevent Blindness, Inc., New York, NY, and the Walter G. Ross Foundation, Washington, DC. The authors are grateful to Dr, John C. Saari for the generous gift of anti-human red/llreen cone oi~in antibody, to Dr, Larry A. Donoso for the kind gift of anti-bovine S-antigen antibody and helpful discussions, and to Dr, Paul A. Hargravef or the generous gift of anti-bovine rhodopsin antibody.",

year = "1996",

month = mar,

day = "11",

doi = "10.1016/0014-5793(96)00163-9",

language = "English (US)",

volume = "382",

pages = "105--110",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Immunolocalization of X-arrestin in human cone photoreceptors

AU - Sakuma, H.

AU - Inana, G.

AU - Murakami, A.

AU - Higashide, T.

AU - McLaren, M. J.

N1 - Funding Information: Acknowledgements: Supported by the Foundation Fighting Blindness, Baltimore, MD, Research to Prevent Blindness, Inc., New York, NY, and the Walter G. Ross Foundation, Washington, DC. The authors are grateful to Dr, John C. Saari for the generous gift of anti-human red/llreen cone oi~in antibody, to Dr, Larry A. Donoso for the kind gift of anti-bovine S-antigen antibody and helpful discussions, and to Dr, Paul A. Hargravef or the generous gift of anti-bovine rhodopsin antibody.

PY - 1996/3/11

Y1 - 1996/3/11

N2 - X-arrestin is a recently identified retina-specific gene of unknown function. Affinity-purified anti-peptide antibody to human X-arrestin was prepared, and used in Western blot analysis of human retinal proteins and for immunohistochemistry on human retinal sections. By Western blot analysis, the antibody specifically bound to an ~47 kDa protein, and by indirect immunofluorescence specifically labeled cone photoreceptors with greatest intensity in their outer segments. In single and double label experiments, the localization of X-arrestin immunoreactivity was compared with immunolabeling patterns obtained with antibodies to red/green cone opsin, rhodopsin, and S-antigen. The results showed that X-arrestin is expressed in red-, green- and blue-sensitive cones in the human retina.

AB - X-arrestin is a recently identified retina-specific gene of unknown function. Affinity-purified anti-peptide antibody to human X-arrestin was prepared, and used in Western blot analysis of human retinal proteins and for immunohistochemistry on human retinal sections. By Western blot analysis, the antibody specifically bound to an ~47 kDa protein, and by indirect immunofluorescence specifically labeled cone photoreceptors with greatest intensity in their outer segments. In single and double label experiments, the localization of X-arrestin immunoreactivity was compared with immunolabeling patterns obtained with antibodies to red/green cone opsin, rhodopsin, and S-antigen. The results showed that X-arrestin is expressed in red-, green- and blue-sensitive cones in the human retina.

KW - Anti-peptide antibody

KW - Arrestin

KW - Human cone photoreceptor

KW - Immunofluorescence

KW - Western blot analysis

KW - X-Arrestin

UR - http://www.scopus.com/inward/record.url?scp=0029945596&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029945596&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(96)00163-9

DO - 10.1016/0014-5793(96)00163-9

M3 - Article

C2 - 8612728

AN - SCOPUS:0029945596

VL - 382

SP - 105

EP - 110

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-2

ER -