Immunolocalization of X-arrestin in human cone photoreceptors

H. Sakuma, G. Inana, A. Murakami, T. Higashide, M. J. McLaren

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

X-arrestin is a recently identified retina-specific gene of unknown function. Affinity-purified anti-peptide antibody to human X-arrestin was prepared, and used in Western blot analysis of human retinal proteins and for immunohistochemistry on human retinal sections. By Western blot analysis, the antibody specifically bound to an ~47 kDa protein, and by indirect immunofluorescence specifically labeled cone photoreceptors with greatest intensity in their outer segments. In single and double label experiments, the localization of X-arrestin immunoreactivity was compared with immunolabeling patterns obtained with antibodies to red/green cone opsin, rhodopsin, and S-antigen. The results showed that X-arrestin is expressed in red-, green- and blue-sensitive cones in the human retina.

Original languageEnglish (US)
Pages (from-to)105-110
Number of pages6
JournalFEBS letters
Volume382
Issue number1-2
DOIs
StatePublished - Mar 11 1996

Keywords

  • Anti-peptide antibody
  • Arrestin
  • Human cone photoreceptor
  • Immunofluorescence
  • Western blot analysis
  • X-Arrestin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Sakuma, H., Inana, G., Murakami, A., Higashide, T., & McLaren, M. J. (1996). Immunolocalization of X-arrestin in human cone photoreceptors. FEBS letters, 382(1-2), 105-110. https://doi.org/10.1016/0014-5793(96)00163-9