Abstract
X-arrestin is a recently identified retina-specific gene of unknown function. Affinity-purified anti-peptide antibody to human X-arrestin was prepared, and used in Western blot analysis of human retinal proteins and for immunohistochemistry on human retinal sections. By Western blot analysis, the antibody specifically bound to an ~47 kDa protein, and by indirect immunofluorescence specifically labeled cone photoreceptors with greatest intensity in their outer segments. In single and double label experiments, the localization of X-arrestin immunoreactivity was compared with immunolabeling patterns obtained with antibodies to red/green cone opsin, rhodopsin, and S-antigen. The results showed that X-arrestin is expressed in red-, green- and blue-sensitive cones in the human retina.
Original language | English (US) |
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Pages (from-to) | 105-110 |
Number of pages | 6 |
Journal | FEBS letters |
Volume | 382 |
Issue number | 1-2 |
DOIs | |
State | Published - Mar 11 1996 |
Keywords
- Anti-peptide antibody
- Arrestin
- Human cone photoreceptor
- Immunofluorescence
- Western blot analysis
- X-Arrestin
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology