Immunolocalization of transforming growth factor α and epidermal growth factor receptor in lungs of patients with cystic fibrosis

William D. Hardie, Pablo A. Bejarano, Mary Ann Miller, James R. Yankaskas, Jon H. Ritter, Jeffrey A. Whitsett, Thomas R. Korfhagen

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Transforming growth factor α (TGF-α) is expressed in respiratory epithelial cells and alveolar macrophages during development and following lung injury. In the present study, the presence and sites of synthesis of TGF-α and its receptor, the epidermal growth factor receptor (EGF-R), were assessed in lung tissue from patients with severe lung disease caused by cystic fibrosis (CF). Lung sections from 24 individuals with CF, obtained at the time of lung transplantation, were compared to lung sections from five lung donors without CF. Cellular sites of TGF-α, EGF-R, and cellular sites of proliferation were assessed by immunohistochemistry. All CF lung sections contained multiple cell types with detectable TGF-α. Compared to control sections, intensity of TGF-α immunostaining in macrophages, airway epithelial cells, and peribronchial submucosal cells was increased. EGF-R was detected in respiratory epithelial and peribronchial stromal cells but not in alveolar macrophages. The intensity of EGF-R staining in CF lung tissue did not differ from that of controls. An increased number of cells expressing Ki- 67 nuclear antigen was detected in peribronchial submucosal cells but not bronchiolar epithelial cells in the CF lungs. The increased expression of TGF-α in CF lung tissue supports the concept that TGF-α plays a role in paracrine/autocrine regulation of lung remodeling associated with injury and repair in the lungs of individuals with cystic fibrosis.

Original languageEnglish
Pages (from-to)415-423
Number of pages9
JournalPediatric and Developmental Pathology
Volume2
Issue number5
DOIs
StatePublished - Sep 1 1999

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Transforming Growth Factors
Epidermal Growth Factor Receptor
Cystic Fibrosis
Lung
Epithelial Cells
Alveolar Macrophages
Ki-67 Antigen
Nuclear Antigens
Growth Factor Receptors
Lung Transplantation
Lung Injury
Stromal Cells
Lung Diseases
Cell Count
Immunohistochemistry
Macrophages
Cell Proliferation
Tissue Donors
Staining and Labeling
Wounds and Injuries

Keywords

  • Cystic fibrosis
  • Epidermal growth factor receptor
  • Transforming growth factor α

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Pathology and Forensic Medicine

Cite this

Hardie, W. D., Bejarano, P. A., Miller, M. A., Yankaskas, J. R., Ritter, J. H., Whitsett, J. A., & Korfhagen, T. R. (1999). Immunolocalization of transforming growth factor α and epidermal growth factor receptor in lungs of patients with cystic fibrosis. Pediatric and Developmental Pathology, 2(5), 415-423. https://doi.org/10.1007/s100249900144

Immunolocalization of transforming growth factor α and epidermal growth factor receptor in lungs of patients with cystic fibrosis. / Hardie, William D.; Bejarano, Pablo A.; Miller, Mary Ann; Yankaskas, James R.; Ritter, Jon H.; Whitsett, Jeffrey A.; Korfhagen, Thomas R.

In: Pediatric and Developmental Pathology, Vol. 2, No. 5, 01.09.1999, p. 415-423.

Research output: Contribution to journalArticle

Hardie, William D. ; Bejarano, Pablo A. ; Miller, Mary Ann ; Yankaskas, James R. ; Ritter, Jon H. ; Whitsett, Jeffrey A. ; Korfhagen, Thomas R. / Immunolocalization of transforming growth factor α and epidermal growth factor receptor in lungs of patients with cystic fibrosis. In: Pediatric and Developmental Pathology. 1999 ; Vol. 2, No. 5. pp. 415-423.
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