Immunolocalization of potassium-chloride cotransporter polypeptides in rat exocrine glands

Eleni Roussa, Boris E. Shmukler, Sabine Wilhelm, Sabina Casula, Alan K. Stuart-Tilley, Frank Thévenod, Seth L. Alper

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Potassium-chloride cotransporters (KCCs) encoded by at least four homologous genes are believed to contribute to cell volume regulation and transepithelial ion transport. We have studied KCC polypeptide expression and immunolocalization of KCCs in rat salivary glands and pancreas. Immunoblot analysis of submandibular, parotid, and pancreas plasma membrane fractions with immunospecific antibodies raised against mouse KCC1 revealed protein bands at ca 135 kDa and ca 150 kDa. Immunocytochemical analysis of fixed salivary and pancreas tissue revealed basolateral KCC1 distribution in rat parotid and pancreatic acinar cells, as well as in parotid, submandibular, and pancreatic duct cells. KCC1 or the polypeptide product(s) of one or more additional KCC genes was also expressed in the basolateral membranes of submandibular acinar cells. Both immunoblot and immunofluorescence signals were abolished in the presence of the peptide antigen. These results establish the presence in rat exocrine glands of KCC1 and likely other KCC polypeptides, and suggest a contribution of KCC polypeptides to transepithelial Cl- transport.

Original languageEnglish (US)
Pages (from-to)335-344
Number of pages10
JournalHistochemistry and Cell Biology
Volume117
Issue number4
DOIs
StatePublished - Sep 17 2002

Keywords

  • Exocrine glands
  • Immunoblot
  • Immunofluorescence microscopy
  • Pancreatic secretion
  • Potassium-chloride cotransport
  • Salivary secretion

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

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    Roussa, E., Shmukler, B. E., Wilhelm, S., Casula, S., Stuart-Tilley, A. K., Thévenod, F., & Alper, S. L. (2002). Immunolocalization of potassium-chloride cotransporter polypeptides in rat exocrine glands. Histochemistry and Cell Biology, 117(4), 335-344. https://doi.org/10.1007/s00418-002-0393-3