Immunolocalization of potassium-chloride cotransporter polypeptides in rat exocrine glands

Eleni Roussa, Boris E. Shmukler, Sabine Wilhelm, Sabina Casula, Alan K. Stuart-Tilley, Frank Thévenod, Seth L. Alper

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Potassium-chloride cotransporters (KCCs) encoded by at least four homologous genes are believed to contribute to cell volume regulation and transepithelial ion transport. We have studied KCC polypeptide expression and immunolocalization of KCCs in rat salivary glands and pancreas. Immunoblot analysis of submandibular, parotid, and pancreas plasma membrane fractions with immunospecific antibodies raised against mouse KCC1 revealed protein bands at ca 135 kDa and ca 150 kDa. Immunocytochemical analysis of fixed salivary and pancreas tissue revealed basolateral KCC1 distribution in rat parotid and pancreatic acinar cells, as well as in parotid, submandibular, and pancreatic duct cells. KCC1 or the polypeptide product(s) of one or more additional KCC genes was also expressed in the basolateral membranes of submandibular acinar cells. Both immunoblot and immunofluorescence signals were abolished in the presence of the peptide antigen. These results establish the presence in rat exocrine glands of KCC1 and likely other KCC polypeptides, and suggest a contribution of KCC polypeptides to transepithelial Cl- transport.

Original languageEnglish
Pages (from-to)335-344
Number of pages10
JournalHistochemistry and Cell Biology
Volume117
Issue number4
DOIs
StatePublished - Sep 17 2002
Externally publishedYes

Fingerprint

Exocrine Glands
glands
potassium chlorides
Polypeptides
polypeptides
pancreas
rats
Potassium
Rats
Peptides
Pancreas
cells
genes
Acinar Cells
Member 4 Solute Carrier Family 12
Genes
membranes
salivary glands
antigens
Cell membranes

Keywords

  • Exocrine glands
  • Immunoblot
  • Immunofluorescence microscopy
  • Pancreatic secretion
  • Potassium-chloride cotransport
  • Salivary secretion

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

Cite this

Roussa, E., Shmukler, B. E., Wilhelm, S., Casula, S., Stuart-Tilley, A. K., Thévenod, F., & Alper, S. L. (2002). Immunolocalization of potassium-chloride cotransporter polypeptides in rat exocrine glands. Histochemistry and Cell Biology, 117(4), 335-344. https://doi.org/10.1007/s00418-002-0393-3

Immunolocalization of potassium-chloride cotransporter polypeptides in rat exocrine glands. / Roussa, Eleni; Shmukler, Boris E.; Wilhelm, Sabine; Casula, Sabina; Stuart-Tilley, Alan K.; Thévenod, Frank; Alper, Seth L.

In: Histochemistry and Cell Biology, Vol. 117, No. 4, 17.09.2002, p. 335-344.

Research output: Contribution to journalArticle

Roussa, E, Shmukler, BE, Wilhelm, S, Casula, S, Stuart-Tilley, AK, Thévenod, F & Alper, SL 2002, 'Immunolocalization of potassium-chloride cotransporter polypeptides in rat exocrine glands', Histochemistry and Cell Biology, vol. 117, no. 4, pp. 335-344. https://doi.org/10.1007/s00418-002-0393-3
Roussa E, Shmukler BE, Wilhelm S, Casula S, Stuart-Tilley AK, Thévenod F et al. Immunolocalization of potassium-chloride cotransporter polypeptides in rat exocrine glands. Histochemistry and Cell Biology. 2002 Sep 17;117(4):335-344. https://doi.org/10.1007/s00418-002-0393-3
Roussa, Eleni ; Shmukler, Boris E. ; Wilhelm, Sabine ; Casula, Sabina ; Stuart-Tilley, Alan K. ; Thévenod, Frank ; Alper, Seth L. / Immunolocalization of potassium-chloride cotransporter polypeptides in rat exocrine glands. In: Histochemistry and Cell Biology. 2002 ; Vol. 117, No. 4. pp. 335-344.
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