Corticotropin releasing factor (CRF)-immunoreactive neurons were detected in the paraventricular nuclei (PVN) of the rat brain, using both the traditional and the recently developed silver-gold intensified PAP methods at light and electron microscopic levels. The latter technique was more sensitive, compared to the classical PAP method, and proved to be highly specific at the ultrastructural level. The immunolabeled perikarya showed smooth or rough contoured fusiform or multipolar shape. Bilateral surgical destruction of PVN caused a gradual decrease in the number of CRF-immunopositive fibers of the median eminence. Following the second post-operative week, CRF-immunoreactivity practically disappeared from this area. In the case of unilateral lesion of PVN, the diminution of immunoreactivity was restricted to the ipsilateral side of the median eminence-pituitary stalk region. Applying the silver-gold intensified PAP method to electron microscopy, the detection of immuno-labeled degenerating fibers became possible, among morphologically similar, densely degenerating, but unlabeled, profiles. This study reports that CRF fibers to the capillary system of the median eminence of the rat originate principally from PVN.
- Immuno-electron microscopy
- Rat brain
- Silver intensified PAP-method
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience