Immunohistochemical study of myelin sheaths formed by oligodendrocytes interacting with dissociated dorsal root ganglion neurons in culture

Francis A. Mithen; Patrick M. Wood; Harish C. Agrawal; Richard P. Bunge

doi:10.1016/0006-8993(83)90469-9

Immunohistochemical study of myelin sheaths formed by oligodendrocytes interacting with dissociated dorsal root ganglion neurons in culture

Francis A. Mithen, Patrick M. Wood, Harish C. Agrawal, Richard P. Bunge

Neurological Surgery

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The addition of central nervous system (CNS) glial cells to dissociated networks of rat dorsal root ganglion neurons in tissue culture provided a useful system for the study of CNS myelin sheath formation. The CNS myelin basic proteins (BP) and proteolipid protein (PLP) were demonstrable in these cultures by immunoperoxidase techniques. Both BP and PLP were detectable in myelinating oligodendrocytes and CNS myelin sheaths. Anti-BP serum and anti-PLP serum were useful immunohistochemical staining reagents for the identification of myelinating oligodendrocytes and CNS myelin sheaths in tissue culture.

Original language	English (US)
Pages (from-to)	63-69
Number of pages	7
Journal	Brain research
Volume	262
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(83)90469-9
State	Published - Feb 28 1983

Keywords

encephalitogenic basic proteins
myelin proteins
neuroglia
proteolipid protein
tissue culture

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

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title = "Immunohistochemical study of myelin sheaths formed by oligodendrocytes interacting with dissociated dorsal root ganglion neurons in culture",

abstract = "The addition of central nervous system (CNS) glial cells to dissociated networks of rat dorsal root ganglion neurons in tissue culture provided a useful system for the study of CNS myelin sheath formation. The CNS myelin basic proteins (BP) and proteolipid protein (PLP) were demonstrable in these cultures by immunoperoxidase techniques. Both BP and PLP were detectable in myelinating oligodendrocytes and CNS myelin sheaths. Anti-BP serum and anti-PLP serum were useful immunohistochemical staining reagents for the identification of myelinating oligodendrocytes and CNS myelin sheaths in tissue culture.",

keywords = "encephalitogenic basic proteins, myelin proteins, neuroglia, proteolipid protein, tissue culture",

author = "Mithen, {Francis A.} and Wood, {Patrick M.} and Agrawal, {Harish C.} and Bunge, {Richard P.}",

note = "Funding Information: We thank Ms. Artree James for her expert technical assistance; Ms. Susan Mantia for her efficient secretarial aid; and Mr. Bob Freund for his professional preparation of the final photographs. We thank Dr. Boyd Hartman for his critical review of this manuscript and his thoughtful suggestions. This work was supported by NSF Grant BNS 7824739 and NIH Grant BNS 13464 (H.C.A.) and by National Multiple Sclerosis Society Grant RG 1118 (P.M.W. and R.P.B.).",

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T1 - Immunohistochemical study of myelin sheaths formed by oligodendrocytes interacting with dissociated dorsal root ganglion neurons in culture

AU - Mithen, Francis A.

AU - Wood, Patrick M.

AU - Agrawal, Harish C.

AU - Bunge, Richard P.

N1 - Funding Information: We thank Ms. Artree James for her expert technical assistance; Ms. Susan Mantia for her efficient secretarial aid; and Mr. Bob Freund for his professional preparation of the final photographs. We thank Dr. Boyd Hartman for his critical review of this manuscript and his thoughtful suggestions. This work was supported by NSF Grant BNS 7824739 and NIH Grant BNS 13464 (H.C.A.) and by National Multiple Sclerosis Society Grant RG 1118 (P.M.W. and R.P.B.).

PY - 1983/2/28

Y1 - 1983/2/28

N2 - The addition of central nervous system (CNS) glial cells to dissociated networks of rat dorsal root ganglion neurons in tissue culture provided a useful system for the study of CNS myelin sheath formation. The CNS myelin basic proteins (BP) and proteolipid protein (PLP) were demonstrable in these cultures by immunoperoxidase techniques. Both BP and PLP were detectable in myelinating oligodendrocytes and CNS myelin sheaths. Anti-BP serum and anti-PLP serum were useful immunohistochemical staining reagents for the identification of myelinating oligodendrocytes and CNS myelin sheaths in tissue culture.

AB - The addition of central nervous system (CNS) glial cells to dissociated networks of rat dorsal root ganglion neurons in tissue culture provided a useful system for the study of CNS myelin sheath formation. The CNS myelin basic proteins (BP) and proteolipid protein (PLP) were demonstrable in these cultures by immunoperoxidase techniques. Both BP and PLP were detectable in myelinating oligodendrocytes and CNS myelin sheaths. Anti-BP serum and anti-PLP serum were useful immunohistochemical staining reagents for the identification of myelinating oligodendrocytes and CNS myelin sheaths in tissue culture.

KW - encephalitogenic basic proteins

KW - myelin proteins

KW - neuroglia

KW - proteolipid protein

KW - tissue culture

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