Immunohistochemical study of myelin sheaths formed by oligodendrocytes interacting with dissociated dorsal root ganglion neurons in culture

Francis A. Mithen, Patrick Wood, Harish C. Agrawal, Richard P. Bunge

Research output: Contribution to journal › Article

14 Citations (Scopus)

Abstract

The addition of central nervous system (CNS) glial cells to dissociated networks of rat dorsal root ganglion neurons in tissue culture provided a useful system for the study of CNS myelin sheath formation. The CNS myelin basic proteins (BP) and proteolipid protein (PLP) were demonstrable in these cultures by immunoperoxidase techniques. Both BP and PLP were detectable in myelinating oligodendrocytes and CNS myelin sheaths. Anti-BP serum and anti-PLP serum were useful immunohistochemical staining reagents for the identification of myelinating oligodendrocytes and CNS myelin sheaths in tissue culture.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalBrain Research
Volume262
Issue number1
DOIs
StatePublished - Feb 28 1983
Externally publishedYes

Fingerprint

Oligodendroglia
Spinal Ganglia
Myelin Sheath
Proteolipids
Central Nervous System
Neurons
Blood Proteins
Culture Techniques
Proteins
Myelin Basic Protein
Immunoenzyme Techniques
Neuroglia
Staining and Labeling

Keywords

  • encephalitogenic basic proteins
  • myelin proteins
  • neuroglia
  • proteolipid protein
  • tissue culture

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Immunohistochemical study of myelin sheaths formed by oligodendrocytes interacting with dissociated dorsal root ganglion neurons in culture. / Mithen, Francis A.; Wood, Patrick; Agrawal, Harish C.; Bunge, Richard P.

In: Brain Research, Vol. 262, No. 1, 28.02.1983, p. 63-69.

Research output: Contribution to journal › Article

Mithen, FA, Wood, P, Agrawal, HC & Bunge, RP 1983, 'Immunohistochemical study of myelin sheaths formed by oligodendrocytes interacting with dissociated dorsal root ganglion neurons in culture', Brain Research, vol. 262, no. 1, pp. 63-69. https://doi.org/10.1016/0006-8993(83)90469-9
Mithen FA, Wood P, Agrawal HC, Bunge RP. Immunohistochemical study of myelin sheaths formed by oligodendrocytes interacting with dissociated dorsal root ganglion neurons in culture. Brain Research. 1983 Feb 28;262(1):63-69. https://doi.org/10.1016/0006-8993(83)90469-9
Mithen, Francis A. ; Wood, Patrick ; Agrawal, Harish C. ; Bunge, Richard P. / Immunohistochemical study of myelin sheaths formed by oligodendrocytes interacting with dissociated dorsal root ganglion neurons in culture. In: Brain Research. 1983 ; Vol. 262, No. 1. pp. 63-69.
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