Abstract
This study aims to illustrate potential transport mechanisms behind the divergent approaches to nitrogen excretion seen in the ureotelic toadfish (Opsanus beta) and the ammoniotelic plainfin midshipman (Porichthys notatus). Specifically, we wish to confirm the expression of a urea transporter (UT), which is found in the gill of the toadfish and which is responsible for the unique "pulsing" nature of urea excretion and to localize the transporter within specific gill cells and at specific cellular locations. Additionally, the localization of ammonia transporters (Rhesus glycoproteins; Rhs) within the gill of both the toadfish and midshipman was explored. Toadfish UT (tUT) was found within Na+-K+-ATPase (NKA)-enriched cells, i.e., ionocytes (probably mitochondria-rich cells), especially along the basolateral membrane and potentially on the apical membrane. In contrast, midshipman UT (pnUT) immunoreactivity did not colocalize with NKA immunoreactivity and was not found along the filaments but instead within the lamellae. The cellular location of Rh proteins was also dissimilar between the two fish species. In toadfish gills, the Rh isoform Rhcg1 was expressed in both NKA-reactive cells and non-reactive cells, whereas Rhbg and Rhcg2 were only expressed in the latter. In contrast, Rhbg, Rhcg1 and Rhcg2 were expressed in both NKA-reactive and non-reactive cells of midshipman gills. In an additional transport epithelium, namely the intestine, the expression of both UTs and Rhs was similar between the two species, with only subtle differences being observed.
| Original language | English |
|---|---|
| Pages (from-to) | 623-637 |
| Number of pages | 15 |
| Journal | Cell and Tissue Research |
| Volume | 352 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 1 2013 |
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Keywords
- Ammonia transporter
- Gill
- Intestine
- Midshipman (Porichthys notatus)
- Nitrogen excretion
- Rhesus glycoprotein
- Toadfish (Opsanus beta)
- Urea transporter
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Cell Biology
- Histology
Cite this
Immunohistochemical localization of urea and ammonia transporters in two confamilial fish species, the ureotelic gulf toadfish (Opsanus beta) and the ammoniotelic plainfin midshipman (Porichthys notatus). / Bucking, Carol; Edwards, Susan L.; Tickle, Paul; Smith, Craig P.; Mcdonald, Danielle M; Walsh, Patrick J.
In: Cell and Tissue Research, Vol. 352, No. 3, 01.06.2013, p. 623-637.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Immunohistochemical localization of urea and ammonia transporters in two confamilial fish species, the ureotelic gulf toadfish (Opsanus beta) and the ammoniotelic plainfin midshipman (Porichthys notatus)
AU - Bucking, Carol
AU - Edwards, Susan L.
AU - Tickle, Paul
AU - Smith, Craig P.
AU - Mcdonald, Danielle M
AU - Walsh, Patrick J.
PY - 2013/6/1
Y1 - 2013/6/1
N2 - This study aims to illustrate potential transport mechanisms behind the divergent approaches to nitrogen excretion seen in the ureotelic toadfish (Opsanus beta) and the ammoniotelic plainfin midshipman (Porichthys notatus). Specifically, we wish to confirm the expression of a urea transporter (UT), which is found in the gill of the toadfish and which is responsible for the unique "pulsing" nature of urea excretion and to localize the transporter within specific gill cells and at specific cellular locations. Additionally, the localization of ammonia transporters (Rhesus glycoproteins; Rhs) within the gill of both the toadfish and midshipman was explored. Toadfish UT (tUT) was found within Na+-K+-ATPase (NKA)-enriched cells, i.e., ionocytes (probably mitochondria-rich cells), especially along the basolateral membrane and potentially on the apical membrane. In contrast, midshipman UT (pnUT) immunoreactivity did not colocalize with NKA immunoreactivity and was not found along the filaments but instead within the lamellae. The cellular location of Rh proteins was also dissimilar between the two fish species. In toadfish gills, the Rh isoform Rhcg1 was expressed in both NKA-reactive cells and non-reactive cells, whereas Rhbg and Rhcg2 were only expressed in the latter. In contrast, Rhbg, Rhcg1 and Rhcg2 were expressed in both NKA-reactive and non-reactive cells of midshipman gills. In an additional transport epithelium, namely the intestine, the expression of both UTs and Rhs was similar between the two species, with only subtle differences being observed.
AB - This study aims to illustrate potential transport mechanisms behind the divergent approaches to nitrogen excretion seen in the ureotelic toadfish (Opsanus beta) and the ammoniotelic plainfin midshipman (Porichthys notatus). Specifically, we wish to confirm the expression of a urea transporter (UT), which is found in the gill of the toadfish and which is responsible for the unique "pulsing" nature of urea excretion and to localize the transporter within specific gill cells and at specific cellular locations. Additionally, the localization of ammonia transporters (Rhesus glycoproteins; Rhs) within the gill of both the toadfish and midshipman was explored. Toadfish UT (tUT) was found within Na+-K+-ATPase (NKA)-enriched cells, i.e., ionocytes (probably mitochondria-rich cells), especially along the basolateral membrane and potentially on the apical membrane. In contrast, midshipman UT (pnUT) immunoreactivity did not colocalize with NKA immunoreactivity and was not found along the filaments but instead within the lamellae. The cellular location of Rh proteins was also dissimilar between the two fish species. In toadfish gills, the Rh isoform Rhcg1 was expressed in both NKA-reactive cells and non-reactive cells, whereas Rhbg and Rhcg2 were only expressed in the latter. In contrast, Rhbg, Rhcg1 and Rhcg2 were expressed in both NKA-reactive and non-reactive cells of midshipman gills. In an additional transport epithelium, namely the intestine, the expression of both UTs and Rhs was similar between the two species, with only subtle differences being observed.
KW - Ammonia transporter
KW - Gill
KW - Intestine
KW - Midshipman (Porichthys notatus)
KW - Nitrogen excretion
KW - Rhesus glycoprotein
KW - Toadfish (Opsanus beta)
KW - Urea transporter
UR - http://www.scopus.com/inward/record.url?scp=84878615893&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84878615893&partnerID=8YFLogxK
U2 - 10.1007/s00441-013-1591-0
DO - 10.1007/s00441-013-1591-0
M3 - Article
C2 - 23512140
AN - SCOPUS:84878615893
VL - 352
SP - 623
EP - 637
JO - Cell and Tissue Research
JF - Cell and Tissue Research
SN - 0302-766X
IS - 3
ER -