Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody

A. Hameed, K. J. Olsen, L. Cheng, W. M. Fox, R. H. Hruban, E. R. Podack

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Perforin is a potent cytolytic pore-forming protein expressed in cytoplasmic granules of cytotoxic T lymphocytes and natural killer cells. A new monoclonal antibody raised against human perforin was used to detect both in vitro and in vivo perforin expression in cytotoxic cells. Immunohistochemical analysis of human peripheral blood mononuclear cells cultured in recombinant interleukin-2 (rIL-2) showed strong granular cytoplasmic staining of the IL-2 activated cytotoxic cells. Fresh-frozen tissue sections from patients with heart allograft rejection were also stained. Strong granular cytoplasmic staining of the mononuclear inflammatory infiltrate characteristic for perforin in cardiac allograft rejection was observed. The detection and quantitative analysis of perforin-associated cytotoxic cells by the human anti-perforin monoclonal antibody will help to evaluate the significance of these functionally distinct cytotoxic cells in human tissue.

Original languageEnglish (US)
Pages (from-to)1025-1030
Number of pages6
JournalAmerican Journal of Pathology
Volume140
Issue number5
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Hameed, A., Olsen, K. J., Cheng, L., Fox, W. M., Hruban, R. H., & Podack, E. R. (1992). Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. American Journal of Pathology, 140(5), 1025-1030.