Immunohistochemical detection of growth hormone-release inhibiting hormone (somatostatin) in the guinea-pig brain

D. Dubé, R. Leclerc, G. Pelletier, A. Arimura, Andrew V Schally

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Utilizing the unlabeled antibody peroxidase-antiperoxidase method, we have studied the distribution of somatostatin (growth hormone release-inhibiting hormone) in the guinea pig brain. An immunohistochemical reaction was found in both internal and external zone of the caudal and median portions of the median eminence. Reaction products were also present in the arcuate nucleus in proximity of the third ventricule. The immunostained elements resembled nerve processes. No cell bodies were stained. A positive reaction was also present in the whole organum vasculosum of the lamina terminalis. In the subfornical and subcommissural organs, somatostatin was observed in the ependymal and subependymal layers and the perivascular areas. In the pineal gland, a positive reaction was also found mainly in the perivascular areas. All the other brain areas were completely negative. These results establish that, in the hypothalamus, somatostatin is mainly concentrated in the median eminence and suggest that periventricular organs can play a role in the regulation of growth hormone secretion.

Original languageEnglish
Pages (from-to)385-392
Number of pages8
JournalCell and Tissue Research
Volume161
Issue number3
DOIs
StatePublished - Aug 1 1975
Externally publishedYes

Fingerprint

Somatostatin
Growth Hormone
Brain
Median Eminence
Guinea Pigs
Hormones
Subcommissural Organ
Subfornical Organ
Arcuate Nucleus of Hypothalamus
Pineal Gland
Reaction products
Peroxidase
Hypothalamus
Cells
Antibodies

Keywords

  • Immunohistochemistry
  • Neuroendocrine function
  • Somatostatin

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Immunohistochemical detection of growth hormone-release inhibiting hormone (somatostatin) in the guinea-pig brain. / Dubé, D.; Leclerc, R.; Pelletier, G.; Arimura, A.; Schally, Andrew V.

In: Cell and Tissue Research, Vol. 161, No. 3, 01.08.1975, p. 385-392.

Research output: Contribution to journalArticle

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