Immunoglobulin A supplementation abrogates bacterial translocation and preserves the architecture of the intestinal epithelium

E. C. Dickinson, J. C. Gorga, M. Garrett, R. Tuncer, P. Boyle, S. C. Watkins, S. M. Alber, M. Parizhskaya, M. Trucco, M. I. Rowe, Henri Ford, B. W. Warner, J. Alverdy

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Background. Breast milk has been shown to prevent gut-origin infections in neonates through undefined mechanisms. Putative protective factors in breast milk include immunoglobulin (Ig)A, IgG, and lactoferrin. We examined their role in bacteriai translocation in neonatal rabbits. Methods, IgA, IgG, and lactoferrin were isolated from rabbit breast milk through gel filtration and ion-exchange chromatography. Neonates were randomized to receive breast milk, formula alone, or formula supplemented with IgA, IgG, or lactoferrin. Quantitative cultures were performed on day 7 for bacterial translocation. Hematoxylin-eosin-stained sections of distal ileum were examined by light microscopy. Transmucosal bacterial passage was determined in vitro, and the ileal mucosal membranes were examined by con focal microscopy. Results. IgA supplementation abrogated bacterial translocation. IgG and lactoferrin had no significant effect. Neonates that received IgA or breast milk gained more weight than those in the other groups. IgA reduced transmucosal bacterial passage in vitro. In contrast to the normal-appearing distal ileum of neonates fed breast milk, intestinal epithelium from neonates that received formula or formula with IgG or IgA demonstrated prominent vacuoles by light microscopy. Those fed formula alone or formula with lactoferrin had slightly shortened villi. Conclusions. IgA supplementation prevents bacterial translocation by enhancing gut mucosal barrier function.

Original languageEnglish (US)
Pages (from-to)284-290
Number of pages7
JournalSurgery
Volume124
Issue number2
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Bacterial Translocation
Intestinal Mucosa
Immunoglobulin A
Human Milk
Lactoferrin
Immunoglobulin G
Microscopy
Ileum
Rabbits
Light
Ion Exchange Chromatography
Hematoxylin
Eosine Yellowish-(YS)
Vacuoles
Gel Chromatography
Weights and Measures
Membranes

ASJC Scopus subject areas

  • Surgery

Cite this

Dickinson, E. C., Gorga, J. C., Garrett, M., Tuncer, R., Boyle, P., Watkins, S. C., ... Alverdy, J. (1998). Immunoglobulin A supplementation abrogates bacterial translocation and preserves the architecture of the intestinal epithelium. Surgery, 124(2), 284-290. https://doi.org/10.1016/S0039-6060(98)70132-1

Immunoglobulin A supplementation abrogates bacterial translocation and preserves the architecture of the intestinal epithelium. / Dickinson, E. C.; Gorga, J. C.; Garrett, M.; Tuncer, R.; Boyle, P.; Watkins, S. C.; Alber, S. M.; Parizhskaya, M.; Trucco, M.; Rowe, M. I.; Ford, Henri; Warner, B. W.; Alverdy, J.

In: Surgery, Vol. 124, No. 2, 01.01.1998, p. 284-290.

Research output: Contribution to journalArticle

Dickinson, EC, Gorga, JC, Garrett, M, Tuncer, R, Boyle, P, Watkins, SC, Alber, SM, Parizhskaya, M, Trucco, M, Rowe, MI, Ford, H, Warner, BW & Alverdy, J 1998, 'Immunoglobulin A supplementation abrogates bacterial translocation and preserves the architecture of the intestinal epithelium', Surgery, vol. 124, no. 2, pp. 284-290. https://doi.org/10.1016/S0039-6060(98)70132-1
Dickinson, E. C. ; Gorga, J. C. ; Garrett, M. ; Tuncer, R. ; Boyle, P. ; Watkins, S. C. ; Alber, S. M. ; Parizhskaya, M. ; Trucco, M. ; Rowe, M. I. ; Ford, Henri ; Warner, B. W. ; Alverdy, J. / Immunoglobulin A supplementation abrogates bacterial translocation and preserves the architecture of the intestinal epithelium. In: Surgery. 1998 ; Vol. 124, No. 2. pp. 284-290.
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abstract = "Background. Breast milk has been shown to prevent gut-origin infections in neonates through undefined mechanisms. Putative protective factors in breast milk include immunoglobulin (Ig)A, IgG, and lactoferrin. We examined their role in bacteriai translocation in neonatal rabbits. Methods, IgA, IgG, and lactoferrin were isolated from rabbit breast milk through gel filtration and ion-exchange chromatography. Neonates were randomized to receive breast milk, formula alone, or formula supplemented with IgA, IgG, or lactoferrin. Quantitative cultures were performed on day 7 for bacterial translocation. Hematoxylin-eosin-stained sections of distal ileum were examined by light microscopy. Transmucosal bacterial passage was determined in vitro, and the ileal mucosal membranes were examined by con focal microscopy. Results. IgA supplementation abrogated bacterial translocation. IgG and lactoferrin had no significant effect. Neonates that received IgA or breast milk gained more weight than those in the other groups. IgA reduced transmucosal bacterial passage in vitro. In contrast to the normal-appearing distal ileum of neonates fed breast milk, intestinal epithelium from neonates that received formula or formula with IgG or IgA demonstrated prominent vacuoles by light microscopy. Those fed formula alone or formula with lactoferrin had slightly shortened villi. Conclusions. IgA supplementation prevents bacterial translocation by enhancing gut mucosal barrier function.",
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