Immunofluorescent study of a chick lens fiber cell membrane polypeptide

Phillip R. Waggoner, Harry Maisel

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

A water-insoluble membrane rich fraction was isolated from the lenses of three-month-old chickens and subjected to electrophoresis on 5·13% polyacrylamide gels containing 1% sodium dodecyl sulfate. The major polypeptide (mol. wt. 26 000 daltons) was removed from several gels and used to immunize rabbits. The specific antiserum was used for the indirect immunofluorescent detection of the first appearance and localization of the major membrane polypeptide in embryonic and post-embryonic chick lenses. The membrane antigen was first detected after four days of incubation in the forming lens fibers just beneath the epithelium and it was restricted to the cell membranes. Thereafter, a positive reaction in the fiber membranes was found throughout the lens fiber mass but never in the epithelium. The lens cell membranes were seen to first become positive in the equatorial zone as the cells began to elongate and form fibers.

Original languageEnglish (US)
Pages (from-to)151-157
Number of pages7
JournalExperimental Eye Research
Volume27
Issue number2
DOIs
StatePublished - Aug 1978

Keywords

  • cell
  • fiber
  • immunofluorescence
  • lens
  • membrane
  • ontogeny
  • polypeptide

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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