Immunofluorescent monoclonal antibody detection of breast cancer in bone marrow

Sensitivity in a model system

M. P. Osborne, S. Asina, G. Y. Wong, L. J. Old, Richard J Cote

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We have previously shown that occult micrometastases can be detected in the bone marrow of breast cancer patients using a panel of epithelial specific monoclonal antibodies in an indirect immunofluorescent assay. The sensitivity of this assay has been examined using cells from an established human breast cancer cell line (MCF-7) mixed with normal bone marrow at various dilutions from 400 cancer cells/106 marrow cells (400:106) to 10:106. MCF-7 cells were detected at the lowest concentration studied, namely 10:106. The number of fluorescent labeled MCF-7 cells counted at each concentration was related to the concentration by a simple nonlinear statistical model. At the concentration of 10:106, the model shows that this technique has the sensitivity to detect between two and four MCF-7 cells 95% of the time. Moreover, by extrapolation, the model predicts that even at the very low concentration of 2:106, there is a 95% chance of detecting one cancer cell. Therefore, this assay may be a very sensitive method for detecting cancer cells in the bone marrow in vivo.

Original languageEnglish
Pages (from-to)2510-2513
Number of pages4
JournalCancer Research
Volume49
Issue number9
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Bone Neoplasms
MCF-7 Cells
Bone Marrow
Monoclonal Antibodies
Breast Neoplasms
Neoplasms
Neoplasm Micrometastasis
Nonlinear Dynamics
Statistical Models
Bone Marrow Cells
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Immunofluorescent monoclonal antibody detection of breast cancer in bone marrow : Sensitivity in a model system. / Osborne, M. P.; Asina, S.; Wong, G. Y.; Old, L. J.; Cote, Richard J.

In: Cancer Research, Vol. 49, No. 9, 01.01.1989, p. 2510-2513.

Research output: Contribution to journalArticle

Osborne, M. P. ; Asina, S. ; Wong, G. Y. ; Old, L. J. ; Cote, Richard J. / Immunofluorescent monoclonal antibody detection of breast cancer in bone marrow : Sensitivity in a model system. In: Cancer Research. 1989 ; Vol. 49, No. 9. pp. 2510-2513.
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