Immunofluorescence findings in human renal amyloidosis

R. Jerath, R. Valenzuela, I. Guerrero, S. D. Deodhar, D. B. Vidt

Research output: Contribution to journalArticle

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Abstract

Positive direct immunofluorescence findings have been occasionally reported for human renal amyloidosis. However, no substantial information describing the frequency or immunomicroscopic pattern has been reported. Twenty-seven renal biopsy specimens with proven amyloidosis were studied by direct immunofluorescence using monospecific antisera against IgG, IgA, IgM, IgD, IgE, kappa and lambda light chains, C3, C4 properdin, alpha-2 macroglobulin, albumin, transferrin, and fibrinogen. Ten biopsy specimens were negative. The remaining specimens showed principally a diffuse immunofluorescent staining of the mesangial areas with various of the above fluorescein-labeled antisera except IgE, albumin, and transferrin antisera, which gave a consistently negative reaction. IgD, properdin, or fibrinogen were weakly identified in only one case. Immunoglobulins and C3 were observed in 14 cases. Kappa and/or lambda light chains were found in nine of ten biopsy specimens so tested. Positive immunofluorescent staining of other elements of the nephron was rarely observed. These results suggest that various immunoreactants, in addition to light chains, can be frequently detected in renal amyloidosis and that passive nonselective absorption of plasma proteins does not explain these immunomicroscopic findings.

Original languageEnglish
Pages (from-to)630-635
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume74
Issue number5
StatePublished - Dec 1 1980
Externally publishedYes

Fingerprint

Amyloidosis
Properdin
Fluorescent Antibody Technique
Immunoglobulin D
Immune Sera
Direct Fluorescent Antibody Technique
Transferrin
Kidney
Biopsy
Light
Fibrinogen
Immunoglobulin E
Albumins
Staining and Labeling
alpha-Macroglobulins
Nephrons
Fluorescein
Immunoglobulin A
Immunoglobulin M
Immunoglobulins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Jerath, R., Valenzuela, R., Guerrero, I., Deodhar, S. D., & Vidt, D. B. (1980). Immunofluorescence findings in human renal amyloidosis. American Journal of Clinical Pathology, 74(5), 630-635.

Immunofluorescence findings in human renal amyloidosis. / Jerath, R.; Valenzuela, R.; Guerrero, I.; Deodhar, S. D.; Vidt, D. B.

In: American Journal of Clinical Pathology, Vol. 74, No. 5, 01.12.1980, p. 630-635.

Research output: Contribution to journalArticle

Jerath, R, Valenzuela, R, Guerrero, I, Deodhar, SD & Vidt, DB 1980, 'Immunofluorescence findings in human renal amyloidosis', American Journal of Clinical Pathology, vol. 74, no. 5, pp. 630-635.
Jerath R, Valenzuela R, Guerrero I, Deodhar SD, Vidt DB. Immunofluorescence findings in human renal amyloidosis. American Journal of Clinical Pathology. 1980 Dec 1;74(5):630-635.
Jerath, R. ; Valenzuela, R. ; Guerrero, I. ; Deodhar, S. D. ; Vidt, D. B. / Immunofluorescence findings in human renal amyloidosis. In: American Journal of Clinical Pathology. 1980 ; Vol. 74, No. 5. pp. 630-635.
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