Immunoelectrophoretic and immunohistochemical characterizations of fibrinogen derivatives in atherosclerotic aortic intimas and vascular prosthesis pseudo-intimas

R. Valenzuela, J. R. Shainoff, P. M. DiBello, D. A. Urbanic, J. M. Anderson, G. R. Matsueda, B. J. Kudryk

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Abstract

Cadaveric aortic intimas with uncomplicated atherosclerosis were examined to determine the distribution and polypeptide chain composition of fibrinogen-related protein. Immunohistochemical staining showed deposits rich in fibrinopeptides A and B. The deposits were usually disseminated throughout intimas of moderate thickness < 0.7 mm, but were distributed focally in elongate patches bounded both lumenally and medially by deposit-free tissue in thick atheromas. Saline extracts generally showed undegraded monomers and dimers by electrophoresis. The residual protein contained Aα and γ-chains that were cross-linked predominantly (>80%) into unresolved high Mr (>200 kd) derivatives, whereas Bβ-chains were left non-cross-linked, as occurs in late stages of cross-linking by transglutaminases. The resolved components had electrophoretic mobilities corresponding to characteristic products of both factor XIIIa and tissue-transglutaminase. A greater incorporation of α- rather than γ-chains into cross-linked products implicated tissue-transglutaminase as contributing heavily. By contrast, vascular graft pseudo-intimas and a cadaveric clot were rich in degraded fibrin devoid of fibrinopeptide A, and cross-linked in patterns typical of XIIIa with γ2 dimers constituting the principal product. The findings indicate that the fibrinogen in the aortic intima is comparatively well protected from thrombin and plasnin, and that much of it is deposited through direct cross-linking by tissue-transglutaminase without being converted to fibrin.

Original languageEnglish (US)
Pages (from-to)861-880
Number of pages20
JournalAmerican Journal of Pathology
Volume141
Issue number4
StatePublished - 1992

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Valenzuela, R., Shainoff, J. R., DiBello, P. M., Urbanic, D. A., Anderson, J. M., Matsueda, G. R., & Kudryk, B. J. (1992). Immunoelectrophoretic and immunohistochemical characterizations of fibrinogen derivatives in atherosclerotic aortic intimas and vascular prosthesis pseudo-intimas. American Journal of Pathology, 141(4), 861-880.