Immunocytochemical survey of putative neurotransmitters in taste buds from Necturus maculosus

J. Welton, R. Taylor, A. J. Porter, Stephen D Roper

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

To investigate synaptic mechanisms in taste buds and collect information about synaptic transmission in these sensory organs, we have examined taste buds of the mudpuppy, Necturus maculosus for the presence of neurotransmitters and neuromodulators. Immunocytochemical staining at the light microscopic level revealed the presence of serotonin-like and cholecystokinin-like (CCK) immunoreactivity in basal cells in the taste bud. Nerve fibers innervating taste buds were immunoreactive for vasoactive intestinal peptide-like (VIP), substance P-like, and calcitonin gene-related peptide-like (CGRP) or compounds closely related to these substances. Immunoreactivity for tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT) in the taste cells and nerve fibers was absent. These data suggest that serotonin, CCK, VIP, substance P, and CGRP are involved in synaptic transmission or neuromodulation in the peripheral organs of taste. No evidence was found for cholinergic or adrenergic mechanisms on the basis of the absence of immunocytochemical staining for key enzymes involved in these two transmitter systems.

Original languageEnglish
Pages (from-to)509-521
Number of pages13
JournalJournal of Comparative Neurology
Volume324
Issue number4
DOIs
StatePublished - Oct 20 1992
Externally publishedYes

Fingerprint

Necturus maculosus
Taste Buds
Neurotransmitter Agents
Calcitonin Gene-Related Peptide
Vasoactive Intestinal Peptide
Cholecystokinin
Substance P
Nerve Fibers
Synaptic Transmission
Serotonin
Necturus
Staining and Labeling
Choline O-Acetyltransferase
Tyrosine 3-Monooxygenase
Adrenergic Agents
Cholinergic Agents
Light
Surveys and Questionnaires
Enzymes

Keywords

  • basal cells
  • gustatory
  • immunohistochemistry
  • mudpuppy
  • synapse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Immunocytochemical survey of putative neurotransmitters in taste buds from Necturus maculosus. / Welton, J.; Taylor, R.; Porter, A. J.; Roper, Stephen D.

In: Journal of Comparative Neurology, Vol. 324, No. 4, 20.10.1992, p. 509-521.

Research output: Contribution to journalArticle

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