Immunocytochemical localization of the dopamine transporter in human brain

Brian J. Ciliax, Genny W. Drash, Julie K. Staley, Sharon Haber, Catherine J. Mobley, Gary W. Miller, Elliott J. Mufson, Deborah C Mash, Allan I. Levey

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

The dopamine transporter (DAT) was localized in normal human brain tissue by light microscopic immunocytochemistry by using highly specific monoclonal antibodies. Regional distribution of DAT was found in areas with established dopaminergic circuitry, e.g., mesostriatal, mesolimbic, and mesocortical pathways. Mesencephalic DAT-immunoreactivity was enriched in the dendrites and cell bodies of neurons in the substantia nigra pars compacta and ventral tegmental area. Staining in the striatum and nucleus accumbens was dense and heterogeneous. Mesocortical DAT immunoreactivity in motor, premotor, anterior cingulate, prefrontal, entorhinal/perirhinal, insular, and visual cortices was detected in scattered varicose and a few nonvaricose fibers. Varicose fibers were relatively enriched in the basolateral and central subnuclei of amygdala, with sparser fibers in lateral and basomedial subnuclei. Double-labeling studies combining DAT and tyrosine hydroxylase (TH) immunostaining in the ventral mesencephalon showed two subpopulations of dopaminergic neurons differentiated by the presence or absence of DAT- immunoreactivity in the A9 and A10 cell groups. In other dopaminergic cell groups (A11, A13-A15), TH-positive hypothalamic neurons showed no detectable DAT-immunoreactivity. However, fine DAT-immunoreactive axons were scattered throughout the hypothalamus, particularly concentrated along the medial border, with more coarse axons present along the lateral border. These findings demonstrate that most mesotelencephalic dopamine neurons of human brain express high levels of DAT throughout their entire somatodendritic and axonal domains, whereas a smaller subpopulation of mesencephalic dopamine cells and all hypothalamic dopamine cell groups examined express little or no DAT. These data indicate that different subpopulations of dopaminergic neurons use different mechanisms to regulate their extracellular dopamine levels.

Original languageEnglish
Pages (from-to)38-56
Number of pages19
JournalJournal of Comparative Neurology
Volume409
Issue number1
DOIs
StatePublished - Jun 21 1999

Fingerprint

Dopamine Plasma Membrane Transport Proteins
Brain
Dopaminergic Neurons
Dopamine
Tyrosine 3-Monooxygenase
Axons
Neurons
Entorhinal Cortex
Ventral Tegmental Area
antineoplaston A10
Gyrus Cinguli
Nucleus Accumbens
Visual Cortex
Dendrites
Mesencephalon
Cerebral Cortex
Hypothalamus
Immunohistochemistry
Monoclonal Antibodies
Staining and Labeling

Keywords

  • Antigen retrieval
  • Distribution
  • Double labeling
  • Immunocytochemistry
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ciliax, B. J., Drash, G. W., Staley, J. K., Haber, S., Mobley, C. J., Miller, G. W., ... Levey, A. I. (1999). Immunocytochemical localization of the dopamine transporter in human brain. Journal of Comparative Neurology, 409(1), 38-56. https://doi.org/10.1002/(SICI)1096-9861(19990621)409:1<38::AID-CNE4>3.0.CO;2-1

Immunocytochemical localization of the dopamine transporter in human brain. / Ciliax, Brian J.; Drash, Genny W.; Staley, Julie K.; Haber, Sharon; Mobley, Catherine J.; Miller, Gary W.; Mufson, Elliott J.; Mash, Deborah C; Levey, Allan I.

In: Journal of Comparative Neurology, Vol. 409, No. 1, 21.06.1999, p. 38-56.

Research output: Contribution to journalArticle

Ciliax, BJ, Drash, GW, Staley, JK, Haber, S, Mobley, CJ, Miller, GW, Mufson, EJ, Mash, DC & Levey, AI 1999, 'Immunocytochemical localization of the dopamine transporter in human brain', Journal of Comparative Neurology, vol. 409, no. 1, pp. 38-56. https://doi.org/10.1002/(SICI)1096-9861(19990621)409:1<38::AID-CNE4>3.0.CO;2-1
Ciliax, Brian J. ; Drash, Genny W. ; Staley, Julie K. ; Haber, Sharon ; Mobley, Catherine J. ; Miller, Gary W. ; Mufson, Elliott J. ; Mash, Deborah C ; Levey, Allan I. / Immunocytochemical localization of the dopamine transporter in human brain. In: Journal of Comparative Neurology. 1999 ; Vol. 409, No. 1. pp. 38-56.
@article{b7c073c7cee94c9d81c987def5f9e7b1,
title = "Immunocytochemical localization of the dopamine transporter in human brain",
abstract = "The dopamine transporter (DAT) was localized in normal human brain tissue by light microscopic immunocytochemistry by using highly specific monoclonal antibodies. Regional distribution of DAT was found in areas with established dopaminergic circuitry, e.g., mesostriatal, mesolimbic, and mesocortical pathways. Mesencephalic DAT-immunoreactivity was enriched in the dendrites and cell bodies of neurons in the substantia nigra pars compacta and ventral tegmental area. Staining in the striatum and nucleus accumbens was dense and heterogeneous. Mesocortical DAT immunoreactivity in motor, premotor, anterior cingulate, prefrontal, entorhinal/perirhinal, insular, and visual cortices was detected in scattered varicose and a few nonvaricose fibers. Varicose fibers were relatively enriched in the basolateral and central subnuclei of amygdala, with sparser fibers in lateral and basomedial subnuclei. Double-labeling studies combining DAT and tyrosine hydroxylase (TH) immunostaining in the ventral mesencephalon showed two subpopulations of dopaminergic neurons differentiated by the presence or absence of DAT- immunoreactivity in the A9 and A10 cell groups. In other dopaminergic cell groups (A11, A13-A15), TH-positive hypothalamic neurons showed no detectable DAT-immunoreactivity. However, fine DAT-immunoreactive axons were scattered throughout the hypothalamus, particularly concentrated along the medial border, with more coarse axons present along the lateral border. These findings demonstrate that most mesotelencephalic dopamine neurons of human brain express high levels of DAT throughout their entire somatodendritic and axonal domains, whereas a smaller subpopulation of mesencephalic dopamine cells and all hypothalamic dopamine cell groups examined express little or no DAT. These data indicate that different subpopulations of dopaminergic neurons use different mechanisms to regulate their extracellular dopamine levels.",
keywords = "Antigen retrieval, Distribution, Double labeling, Immunocytochemistry, Tyrosine hydroxylase",
author = "Ciliax, {Brian J.} and Drash, {Genny W.} and Staley, {Julie K.} and Sharon Haber and Mobley, {Catherine J.} and Miller, {Gary W.} and Mufson, {Elliott J.} and Mash, {Deborah C} and Levey, {Allan I.}",
year = "1999",
month = "6",
day = "21",
doi = "10.1002/(SICI)1096-9861(19990621)409:1<38::AID-CNE4>3.0.CO;2-1",
language = "English",
volume = "409",
pages = "38--56",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Immunocytochemical localization of the dopamine transporter in human brain

AU - Ciliax, Brian J.

AU - Drash, Genny W.

AU - Staley, Julie K.

AU - Haber, Sharon

AU - Mobley, Catherine J.

AU - Miller, Gary W.

AU - Mufson, Elliott J.

AU - Mash, Deborah C

AU - Levey, Allan I.

PY - 1999/6/21

Y1 - 1999/6/21

N2 - The dopamine transporter (DAT) was localized in normal human brain tissue by light microscopic immunocytochemistry by using highly specific monoclonal antibodies. Regional distribution of DAT was found in areas with established dopaminergic circuitry, e.g., mesostriatal, mesolimbic, and mesocortical pathways. Mesencephalic DAT-immunoreactivity was enriched in the dendrites and cell bodies of neurons in the substantia nigra pars compacta and ventral tegmental area. Staining in the striatum and nucleus accumbens was dense and heterogeneous. Mesocortical DAT immunoreactivity in motor, premotor, anterior cingulate, prefrontal, entorhinal/perirhinal, insular, and visual cortices was detected in scattered varicose and a few nonvaricose fibers. Varicose fibers were relatively enriched in the basolateral and central subnuclei of amygdala, with sparser fibers in lateral and basomedial subnuclei. Double-labeling studies combining DAT and tyrosine hydroxylase (TH) immunostaining in the ventral mesencephalon showed two subpopulations of dopaminergic neurons differentiated by the presence or absence of DAT- immunoreactivity in the A9 and A10 cell groups. In other dopaminergic cell groups (A11, A13-A15), TH-positive hypothalamic neurons showed no detectable DAT-immunoreactivity. However, fine DAT-immunoreactive axons were scattered throughout the hypothalamus, particularly concentrated along the medial border, with more coarse axons present along the lateral border. These findings demonstrate that most mesotelencephalic dopamine neurons of human brain express high levels of DAT throughout their entire somatodendritic and axonal domains, whereas a smaller subpopulation of mesencephalic dopamine cells and all hypothalamic dopamine cell groups examined express little or no DAT. These data indicate that different subpopulations of dopaminergic neurons use different mechanisms to regulate their extracellular dopamine levels.

AB - The dopamine transporter (DAT) was localized in normal human brain tissue by light microscopic immunocytochemistry by using highly specific monoclonal antibodies. Regional distribution of DAT was found in areas with established dopaminergic circuitry, e.g., mesostriatal, mesolimbic, and mesocortical pathways. Mesencephalic DAT-immunoreactivity was enriched in the dendrites and cell bodies of neurons in the substantia nigra pars compacta and ventral tegmental area. Staining in the striatum and nucleus accumbens was dense and heterogeneous. Mesocortical DAT immunoreactivity in motor, premotor, anterior cingulate, prefrontal, entorhinal/perirhinal, insular, and visual cortices was detected in scattered varicose and a few nonvaricose fibers. Varicose fibers were relatively enriched in the basolateral and central subnuclei of amygdala, with sparser fibers in lateral and basomedial subnuclei. Double-labeling studies combining DAT and tyrosine hydroxylase (TH) immunostaining in the ventral mesencephalon showed two subpopulations of dopaminergic neurons differentiated by the presence or absence of DAT- immunoreactivity in the A9 and A10 cell groups. In other dopaminergic cell groups (A11, A13-A15), TH-positive hypothalamic neurons showed no detectable DAT-immunoreactivity. However, fine DAT-immunoreactive axons were scattered throughout the hypothalamus, particularly concentrated along the medial border, with more coarse axons present along the lateral border. These findings demonstrate that most mesotelencephalic dopamine neurons of human brain express high levels of DAT throughout their entire somatodendritic and axonal domains, whereas a smaller subpopulation of mesencephalic dopamine cells and all hypothalamic dopamine cell groups examined express little or no DAT. These data indicate that different subpopulations of dopaminergic neurons use different mechanisms to regulate their extracellular dopamine levels.

KW - Antigen retrieval

KW - Distribution

KW - Double labeling

KW - Immunocytochemistry

KW - Tyrosine hydroxylase

UR - http://www.scopus.com/inward/record.url?scp=0033591616&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033591616&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9861(19990621)409:1<38::AID-CNE4>3.0.CO;2-1

DO - 10.1002/(SICI)1096-9861(19990621)409:1<38::AID-CNE4>3.0.CO;2-1

M3 - Article

C2 - 10363710

AN - SCOPUS:0033591616

VL - 409

SP - 38

EP - 56

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 1

ER -