Immunocytochemical localization of neurofilaments in the fibre systems of the developing rat spinal cord white matter

Martin Oudega, Johan H C Voormolen, Kamen G. Usunoff, Enrico Marani, Raph T W M Thomeer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The appearance and localization of the protein subunits of neurofilaments in the ascending and descending fibre systems of the developing rat spinal cord white matter were studied. The monoclonal antibody NF-90 (specific for the phosphorylated NF-L, NF-M and NF-H subunits) was used as neurofilament marker in fresh cryostat and Bouin fixed paraffin sections. The results were compared with Nissl and Bodian stained sections. Within the white matter, phosphorylated neurofilament proteins were expressed with regional variations. At embryonal day 12 (E12), the first positive fibres were found in the lateral funiculus. During further development, the peripheral region of the lateral funiculus showed an intense neurofilament staining, due to the presence of a higher number of fibres. From postnatal day 12 (P12) on, an increased amount of neurofilaments was found in the region close to the periphery, probably due to the presence of large calibre fibres. The dorsolateral part of the lateral funiculus filled in with fibres after birth, which indicated the extended development of the rubrospinal tract. At E13, positive fibres were present in the ventral commissure and the ventral funiculus. At E14, an increased amount of neurofilaments was detected in the periphery of the ventral funiculus. At maturity, an intense staining in the subsurface region could be found, due to the presence of large calibre fibres of the fasciculus longitudinalis medialis. At E13, the first neurofilament positive fibres were present in the dorsal funiculus. At this day, a concentration of fibres was found in the dorsal part of the dorsal root bifurcation zone and three days later, more fibres were detected in the medial part of the dorsal funiculus. At E18, a higher number of fibres was present in the dorsal region of the fasciculus gracilis. The mature fasciculus cuneatus showed an intense neurofilament staining, which was mainly present in large calibre fibres. The ventral part of the dorsal funiculus filled in with neurofilament positive fibres after birth. This indicated the relative late arrival of the corticospinal tract.

Original languageEnglish (US)
Pages (from-to)329-346
Number of pages18
JournalEuropean Journal of Morphology
Volume34
Issue number5
DOIs
StatePublished - 1996

Fingerprint

Intermediate Filaments
spinal cord
Spinal Cord
rats
protein subunits
alkanes
monoclonal antibodies
rhizosphere
Staining and Labeling
Parturition
Neurofilament Proteins
Pyramidal Tracts
White Matter
staining
Spinal Nerve Roots
Protein Subunits
proteins
Paraffin
Monoclonal Antibodies

Keywords

  • development
  • immunocytochemistry
  • neuronal cytoskeleton
  • rat
  • spinal cord

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Immunocytochemical localization of neurofilaments in the fibre systems of the developing rat spinal cord white matter. / Oudega, Martin; Voormolen, Johan H C; Usunoff, Kamen G.; Marani, Enrico; Thomeer, Raph T W M.

In: European Journal of Morphology, Vol. 34, No. 5, 1996, p. 329-346.

Research output: Contribution to journalArticle

Oudega, Martin ; Voormolen, Johan H C ; Usunoff, Kamen G. ; Marani, Enrico ; Thomeer, Raph T W M. / Immunocytochemical localization of neurofilaments in the fibre systems of the developing rat spinal cord white matter. In: European Journal of Morphology. 1996 ; Vol. 34, No. 5. pp. 329-346.
@article{658d582df526419a8c2e19f79e05629c,
title = "Immunocytochemical localization of neurofilaments in the fibre systems of the developing rat spinal cord white matter",
abstract = "The appearance and localization of the protein subunits of neurofilaments in the ascending and descending fibre systems of the developing rat spinal cord white matter were studied. The monoclonal antibody NF-90 (specific for the phosphorylated NF-L, NF-M and NF-H subunits) was used as neurofilament marker in fresh cryostat and Bouin fixed paraffin sections. The results were compared with Nissl and Bodian stained sections. Within the white matter, phosphorylated neurofilament proteins were expressed with regional variations. At embryonal day 12 (E12), the first positive fibres were found in the lateral funiculus. During further development, the peripheral region of the lateral funiculus showed an intense neurofilament staining, due to the presence of a higher number of fibres. From postnatal day 12 (P12) on, an increased amount of neurofilaments was found in the region close to the periphery, probably due to the presence of large calibre fibres. The dorsolateral part of the lateral funiculus filled in with fibres after birth, which indicated the extended development of the rubrospinal tract. At E13, positive fibres were present in the ventral commissure and the ventral funiculus. At E14, an increased amount of neurofilaments was detected in the periphery of the ventral funiculus. At maturity, an intense staining in the subsurface region could be found, due to the presence of large calibre fibres of the fasciculus longitudinalis medialis. At E13, the first neurofilament positive fibres were present in the dorsal funiculus. At this day, a concentration of fibres was found in the dorsal part of the dorsal root bifurcation zone and three days later, more fibres were detected in the medial part of the dorsal funiculus. At E18, a higher number of fibres was present in the dorsal region of the fasciculus gracilis. The mature fasciculus cuneatus showed an intense neurofilament staining, which was mainly present in large calibre fibres. The ventral part of the dorsal funiculus filled in with neurofilament positive fibres after birth. This indicated the relative late arrival of the corticospinal tract.",
keywords = "development, immunocytochemistry, neuronal cytoskeleton, rat, spinal cord",
author = "Martin Oudega and Voormolen, {Johan H C} and Usunoff, {Kamen G.} and Enrico Marani and Thomeer, {Raph T W M}",
year = "1996",
doi = "10.1076/ejom.34.5.329.13052",
language = "English (US)",
volume = "34",
pages = "329--346",
journal = "European Journal of Morphology",
issn = "0924-3860",
publisher = "Swets & Zeitlinger",
number = "5",

}

TY - JOUR

T1 - Immunocytochemical localization of neurofilaments in the fibre systems of the developing rat spinal cord white matter

AU - Oudega, Martin

AU - Voormolen, Johan H C

AU - Usunoff, Kamen G.

AU - Marani, Enrico

AU - Thomeer, Raph T W M

PY - 1996

Y1 - 1996

N2 - The appearance and localization of the protein subunits of neurofilaments in the ascending and descending fibre systems of the developing rat spinal cord white matter were studied. The monoclonal antibody NF-90 (specific for the phosphorylated NF-L, NF-M and NF-H subunits) was used as neurofilament marker in fresh cryostat and Bouin fixed paraffin sections. The results were compared with Nissl and Bodian stained sections. Within the white matter, phosphorylated neurofilament proteins were expressed with regional variations. At embryonal day 12 (E12), the first positive fibres were found in the lateral funiculus. During further development, the peripheral region of the lateral funiculus showed an intense neurofilament staining, due to the presence of a higher number of fibres. From postnatal day 12 (P12) on, an increased amount of neurofilaments was found in the region close to the periphery, probably due to the presence of large calibre fibres. The dorsolateral part of the lateral funiculus filled in with fibres after birth, which indicated the extended development of the rubrospinal tract. At E13, positive fibres were present in the ventral commissure and the ventral funiculus. At E14, an increased amount of neurofilaments was detected in the periphery of the ventral funiculus. At maturity, an intense staining in the subsurface region could be found, due to the presence of large calibre fibres of the fasciculus longitudinalis medialis. At E13, the first neurofilament positive fibres were present in the dorsal funiculus. At this day, a concentration of fibres was found in the dorsal part of the dorsal root bifurcation zone and three days later, more fibres were detected in the medial part of the dorsal funiculus. At E18, a higher number of fibres was present in the dorsal region of the fasciculus gracilis. The mature fasciculus cuneatus showed an intense neurofilament staining, which was mainly present in large calibre fibres. The ventral part of the dorsal funiculus filled in with neurofilament positive fibres after birth. This indicated the relative late arrival of the corticospinal tract.

AB - The appearance and localization of the protein subunits of neurofilaments in the ascending and descending fibre systems of the developing rat spinal cord white matter were studied. The monoclonal antibody NF-90 (specific for the phosphorylated NF-L, NF-M and NF-H subunits) was used as neurofilament marker in fresh cryostat and Bouin fixed paraffin sections. The results were compared with Nissl and Bodian stained sections. Within the white matter, phosphorylated neurofilament proteins were expressed with regional variations. At embryonal day 12 (E12), the first positive fibres were found in the lateral funiculus. During further development, the peripheral region of the lateral funiculus showed an intense neurofilament staining, due to the presence of a higher number of fibres. From postnatal day 12 (P12) on, an increased amount of neurofilaments was found in the region close to the periphery, probably due to the presence of large calibre fibres. The dorsolateral part of the lateral funiculus filled in with fibres after birth, which indicated the extended development of the rubrospinal tract. At E13, positive fibres were present in the ventral commissure and the ventral funiculus. At E14, an increased amount of neurofilaments was detected in the periphery of the ventral funiculus. At maturity, an intense staining in the subsurface region could be found, due to the presence of large calibre fibres of the fasciculus longitudinalis medialis. At E13, the first neurofilament positive fibres were present in the dorsal funiculus. At this day, a concentration of fibres was found in the dorsal part of the dorsal root bifurcation zone and three days later, more fibres were detected in the medial part of the dorsal funiculus. At E18, a higher number of fibres was present in the dorsal region of the fasciculus gracilis. The mature fasciculus cuneatus showed an intense neurofilament staining, which was mainly present in large calibre fibres. The ventral part of the dorsal funiculus filled in with neurofilament positive fibres after birth. This indicated the relative late arrival of the corticospinal tract.

KW - development

KW - immunocytochemistry

KW - neuronal cytoskeleton

KW - rat

KW - spinal cord

UR - http://www.scopus.com/inward/record.url?scp=0030512022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030512022&partnerID=8YFLogxK

U2 - 10.1076/ejom.34.5.329.13052

DO - 10.1076/ejom.34.5.329.13052

M3 - Article

C2 - 9034790

AN - SCOPUS:0030512022

VL - 34

SP - 329

EP - 346

JO - European Journal of Morphology

JF - European Journal of Morphology

SN - 0924-3860

IS - 5

ER -