Immunochemical studies of infectious mononucleosis-X. Characterization of a glycoprotein from horse erythrocytes which reacts with Paul-Bunnell antibody

Karen E. Caldwell, Marilyn Cayer, Philip L. Whitney, Mary Ann Fletcher

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A highly purified preparation of horse erythrocyte glycoprotein was prepared from an aqueous ethanolic extract of hemoglobin-free membranes. The subunit apparent mol. wt was 30,000. In aqueous solution the glycoprotein formed globular aggregates of 93 ± 16 Å diameter. The glycoprotein had a receptor for the Paul-Bunnell antibody of infectious mononucleosis which was associated with an O-glycosidically linked oligosaccharide and dependent on the presence of N-glycolylneuraminic acid. In addition the glycoprotein had a neuraminidase-sensitive receptor for human peripheral blood lymphocytes. Fifty per cent inhibition of the rosetting of sheep red cells by 4 × 105 lymphocytes was caused by 30 μg of glycoprotein.

Original languageEnglish
Pages (from-to)779-791
Number of pages13
JournalMolecular Immunology
Volume19
Issue number6
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

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Heterophile Antibodies
Infectious Mononucleosis
Horses
Glycoproteins
Erythrocytes
Lymphocytes
Neuraminidase
Oligosaccharides
Sheep
Hemoglobins
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Immunochemical studies of infectious mononucleosis-X. Characterization of a glycoprotein from horse erythrocytes which reacts with Paul-Bunnell antibody. / Caldwell, Karen E.; Cayer, Marilyn; Whitney, Philip L.; Fletcher, Mary Ann.

In: Molecular Immunology, Vol. 19, No. 6, 01.01.1982, p. 779-791.

Research output: Contribution to journalArticle

Caldwell, Karen E. ; Cayer, Marilyn ; Whitney, Philip L. ; Fletcher, Mary Ann. / Immunochemical studies of infectious mononucleosis-X. Characterization of a glycoprotein from horse erythrocytes which reacts with Paul-Bunnell antibody. In: Molecular Immunology. 1982 ; Vol. 19, No. 6. pp. 779-791.
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