Immunochemical studies of infectious mononucleosis. VI. A radioimmunoassay for the detection of infectious mononucleosis heterophile antibody and antigen

Mary Ann Fletcher; Timothy M. Lo; Barbara A. Levey; William R. Graves

doi:10.1016/S0022-1759(97)90021-1

Immunochemical studies of infectious mononucleosis. VI. A radioimmunoassay for the detection of infectious mononucleosis heterophile antibody and antigen

Mary Ann Fletcher, Timothy M. Lo, Barbara A. Levey, William R. Graves

Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The coated-tube method of solid-phase radioimmunoassay has been adapted to the detection of heterophile antibodies and antigens of infectious mononucleosis. Disposable plastic hemagglutination trays were coated with purified glycoprotein from horse erythrocytes and the subsequent uptake of antibody from test sera was detected by radioiodinated horse erythrocyte glycoprotein. In a preliminary survey of sera from patients with infectious mononucleosis and sera from controls, the assay proved highly sensitive and specific. The test system was also useful in a competitive binding assay for immuno-chemical studies of glycoproteins from other heterophile antigen-positive species.

Original language	English (US)
Pages (from-to)	51-58
Number of pages	8
Journal	Journal of Immunological Methods
Volume	14
Issue number	1
DOIs	https://doi.org/10.1016/S0022-1759(97)90021-1
State	Published - Jan 1977

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/S0022-1759(97)90021-1

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Immunochemical studies of infectious mononucleosis. VI. A radioimmunoassay for the detection of infectious mononucleosis heterophile antibody and antigen. / Fletcher, Mary Ann; Lo, Timothy M.; Levey, Barbara A.; Graves, William R.

In: Journal of Immunological Methods, Vol. 14, No. 1, 01.1977, p. 51-58.

Research output: Contribution to journal › Article › peer-review

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abstract = "The coated-tube method of solid-phase radioimmunoassay has been adapted to the detection of heterophile antibodies and antigens of infectious mononucleosis. Disposable plastic hemagglutination trays were coated with purified glycoprotein from horse erythrocytes and the subsequent uptake of antibody from test sera was detected by radioiodinated horse erythrocyte glycoprotein. In a preliminary survey of sera from patients with infectious mononucleosis and sera from controls, the assay proved highly sensitive and specific. The test system was also useful in a competitive binding assay for immuno-chemical studies of glycoproteins from other heterophile antigen-positive species.",

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note = "Funding Information: We are grateful to Dr. Eugene Flipse, Director of Health Center Hospital, for making available to us the sera from patients with infectious mononucleosis. This work was supported by NIH Grant AM 16763.",

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Immunochemical studies of infectious mononucleosis. VI. A radioimmunoassay for the detection of infectious mononucleosis heterophile antibody and antigen

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