Immunochemical studies of infectious mononucleosis. VI. A radioimmunoassay for the detection of infectious mononucleosis heterophile antibody and antigen

Mary Ann Fletcher, Timothy M. Lo, Barbara A. Levey, William R. Graves

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The coated-tube method of solid-phase radioimmunoassay has been adapted to the detection of heterophile antibodies and antigens of infectious mononucleosis. Disposable plastic hemagglutination trays were coated with purified glycoprotein from horse erythrocytes and the subsequent uptake of antibody from test sera was detected by radioiodinated horse erythrocyte glycoprotein. In a preliminary survey of sera from patients with infectious mononucleosis and sera from controls, the assay proved highly sensitive and specific. The test system was also useful in a competitive binding assay for immuno-chemical studies of glycoproteins from other heterophile antigen-positive species.

Original languageEnglish (US)
Pages (from-to)51-58
Number of pages8
JournalJournal of Immunological Methods
Volume14
Issue number1
DOIs
StatePublished - Jan 1977

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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