Immunochemical Studies of Infectious Mononucleosis IV. Effect of Proteases on the Glycoprotein of Horse Erythrocytes

Mary Ann Fletcher, Timothy M. lo

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The effects of 3 proteases, trypsin, papain, and pronase, upon the major glycoprotein of horse erythrocyte membranes were examined. By 3 independent criteria, papain was the most effective. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of stroma derived from papain treated erythrocytes revealed the loss of the major glycoprotein band characteristic of this membrane. Concomitant with the loss of the major glycoprotein band, fragments were released from the erythrocyte which had a carbohydrate composition similar to that of the intact glycoprotein. Also, reactivity of the treated erythrocytes with infectious mononucleosis antibody, a property associated with the major glycoprotein, was reduced. Only a portion of the membrane glycoprotein was susceptible to pronase action and none was cleaved by trypsin.

Original languageEnglish (US)
Pages (from-to)1100-1105
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume145
Issue number3
DOIs
StatePublished - Mar 1974

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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