Immunochemical studies of infectious mononucleosis. III. Isolation and characterization of heterophile antibodies

M. A. Fletcher, T. M. Lo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The heterophile antibody of infectious mononucleosis was isolated using an immunoabsorbent containing highly purified glycoprotein from horse erythrocyte membrane. Only the IgM fraction of this antibody agglutinated sheep erythrocytes or precipitated with the heterophile antigens from sheep, ox or horse red cells. The IgM antibody contained both kappa and lambda light chain determinants. The only reactivity which could be attributed to the IgG fraction of infectious mononucleosis serum was a slight degree of antigen binding by indirect radioimmunoelectrophoresis using 131I labelled horse erythrocyte glycoprotein.

Original languageEnglish
Pages (from-to)607-614
Number of pages8
JournalClinical and Experimental Immunology
Volume16
Issue number4
StatePublished - Dec 1 1974
Externally publishedYes

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Heterophile Antibodies
Infectious Mononucleosis
Horses
Immunoglobulin M
Sheep
Glycoproteins
Erythrocytes
Heterophile Antigens
Antibodies
Erythrocyte Membrane
Immunoglobulin G
Light
Antigens
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Immunochemical studies of infectious mononucleosis. III. Isolation and characterization of heterophile antibodies. / Fletcher, M. A.; Lo, T. M.

In: Clinical and Experimental Immunology, Vol. 16, No. 4, 01.12.1974, p. 607-614.

Research output: Contribution to journalArticle

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