Immunochemical demonstration of human chorionic gonadotrophin (hCG) in tissue of breast carcinoma

A. Castro, P. Buschbaum, Mehrdad Nadji, W. Voigt, S. Tabei, Azorides R Morales

Research output: Contribution to journalArticle

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Abstract

Immunoreactive human chorionic gonadotrophin (hCG) was found in 9 of 65 surgically removed malignant breast tumours. The concentrations found ranged from 5 to greater than 500 mIU hCG/g tumour. Of the nine, seven extracts were assayed for total protein content; when expressed as mIU/mg extract protein, the hCG concentrations ranged from 0.28 -> 18.2 mIU/mg protein. Comparison of oestrogen receptor and hCG presence in the tumours showed no strong correlation. All tumours in which hCG was detected were shown to be of ductal origin. The hCG was measured by a β-chain specific radioimmunoassay which measures only hCG even in the presence of high levels of other gonadotrophin hormones. In further study of these specimens an immunoperoxidase staining technique was used to stain for hCG in formalin-fixed sections of the same tumours assayed by RIA. This technique confirmed hCG in the positive specimens examined (tissue was available for 7 of the 9 positives) and no significant staining in any negatives. The hCG was shown to be localized within the cytoplasm and on the surface of the malignant cells but not in surrounding normal cells. This intracellular localization demonstrates that the malignant cells may produce hCG, rather than stimulating the pituitary to do so.

Original languageEnglish
Pages (from-to)511-516
Number of pages6
JournalActa Endocrinologica
Volume94
Issue number4
StatePublished - Dec 1 1980

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Chorionic Gonadotropin
Breast Neoplasms
Neoplasms
Staining and Labeling
Proteins
Gonadotropins
Immunoenzyme Techniques
Estrogen Receptors
Formaldehyde
Radioimmunoassay
Cytoplasm
Coloring Agents
Hormones

ASJC Scopus subject areas

  • Endocrinology

Cite this

Castro, A., Buschbaum, P., Nadji, M., Voigt, W., Tabei, S., & Morales, A. R. (1980). Immunochemical demonstration of human chorionic gonadotrophin (hCG) in tissue of breast carcinoma. Acta Endocrinologica, 94(4), 511-516.

Immunochemical demonstration of human chorionic gonadotrophin (hCG) in tissue of breast carcinoma. / Castro, A.; Buschbaum, P.; Nadji, Mehrdad; Voigt, W.; Tabei, S.; Morales, Azorides R.

In: Acta Endocrinologica, Vol. 94, No. 4, 01.12.1980, p. 511-516.

Research output: Contribution to journalArticle

Castro, A, Buschbaum, P, Nadji, M, Voigt, W, Tabei, S & Morales, AR 1980, 'Immunochemical demonstration of human chorionic gonadotrophin (hCG) in tissue of breast carcinoma', Acta Endocrinologica, vol. 94, no. 4, pp. 511-516.
Castro, A. ; Buschbaum, P. ; Nadji, Mehrdad ; Voigt, W. ; Tabei, S. ; Morales, Azorides R. / Immunochemical demonstration of human chorionic gonadotrophin (hCG) in tissue of breast carcinoma. In: Acta Endocrinologica. 1980 ; Vol. 94, No. 4. pp. 511-516.
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