Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes

Dongmei Han, Carlos A. Leyva, Della Matheson, Davide Mineo, Shari Messinger, Bonnie B. Blomberg, Ana Hernandez, Luigi F. Meneghini, Gloria Allende, Jay S. Skyler, Rodolfo Alejandro, Alberto Pugliese, Norma S. Kenyon

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

There is a need for biomarkers to monitor the development and progression of type 1 DM. We analyzed mRNA expression levels for granzyme B, perforin, fas ligand, TNF-αalpha;, IFN-γgamma;, Foxp3, IL-10, TGF-βbeta;, IL-4, IL-6, IL-17, Activation-induced cytidine deaminase (AID) and Immunoglobulin G gamma chain (IgG<gamma>) genes in peripheral blood of at-risk, new-onset and long-term type 1 DM , and healthy controls. The majority of the genes were suppressed in long-term type 1 DM compared to controls and new-onset patients. IFN-γ, IL-4 and IL-10 mRNA levels were significantly higher in new-onset compared to at-risk and long-term groups. There was decreased mRNA expression for AID and IgG<gamma> and up-regulation of IFN-γ with age in controls. Data suggest an overall depressed immunity in long-term type 1 DM. Increased gene expression levels for IFN-γ, IL-4 and IL-10 in new-onset patients from at-risk patients might be used as potential markers for progression of the disease.

Original languageEnglish (US)
Pages (from-to)290-301
Number of pages12
JournalClinical Immunology
Volume139
Issue number3
DOIs
StatePublished - Jun 2011

Keywords

  • Age
  • Biomarkers
  • Cytokines
  • Cytotoxic lymphocyte genes
  • Gene expression
  • Type 1 diabetes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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