Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes

Dongmei Han, Carlos A. Leyva, Della Matheson, Davide Mineo, Shari Messinger, Bonnie B Blomberg, Ana Hernandez, Luigi F. Meneghini, Gloria Allende, Jay S Skyler, Rodolfo Alejandro, Alberto Pugliese, Norma S Kenyon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

There is a need for biomarkers to monitor the development and progression of type 1 DM. We analyzed mRNA expression levels for granzyme B, perforin, fas ligand, TNF-αalpha;, IFN-γgamma;, Foxp3, IL-10, TGF-βbeta;, IL-4, IL-6, IL-17, Activation-induced cytidine deaminase (AID) and Immunoglobulin G gamma chain (IgG<gamma>) genes in peripheral blood of at-risk, new-onset and long-term type 1 DM , and healthy controls. The majority of the genes were suppressed in long-term type 1 DM compared to controls and new-onset patients. IFN-γ, IL-4 and IL-10 mRNA levels were significantly higher in new-onset compared to at-risk and long-term groups. There was decreased mRNA expression for AID and IgG<gamma> and up-regulation of IFN-γ with age in controls. Data suggest an overall depressed immunity in long-term type 1 DM. Increased gene expression levels for IFN-γ, IL-4 and IL-10 in new-onset patients from at-risk patients might be used as potential markers for progression of the disease.

Original languageEnglish
Pages (from-to)290-301
Number of pages12
JournalClinical Immunology
Volume139
Issue number3
DOIs
StatePublished - Jun 1 2011

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Immunoglobulin gamma-Chains
Gene Expression Profiling
Type 1 Diabetes Mellitus
Interleukin-4
Interleukin-10
Messenger RNA
Immunoglobulin G
Fas Ligand Protein
Interleukin-17
Transforming Growth Factor beta
Genes
Disease Progression
Immunity
Interleukin-6
Up-Regulation
Tumor Necrosis Factor-alpha
Biomarkers
Gene Expression
AICDA (activation-induced cytidine deaminase)

Keywords

  • Age
  • Biomarkers
  • Cytokines
  • Cytotoxic lymphocyte genes
  • Gene expression
  • Type 1 diabetes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes. / Han, Dongmei; Leyva, Carlos A.; Matheson, Della; Mineo, Davide; Messinger, Shari; Blomberg, Bonnie B; Hernandez, Ana; Meneghini, Luigi F.; Allende, Gloria; Skyler, Jay S; Alejandro, Rodolfo; Pugliese, Alberto; Kenyon, Norma S.

In: Clinical Immunology, Vol. 139, No. 3, 01.06.2011, p. 290-301.

Research output: Contribution to journalArticle

Han, Dongmei ; Leyva, Carlos A. ; Matheson, Della ; Mineo, Davide ; Messinger, Shari ; Blomberg, Bonnie B ; Hernandez, Ana ; Meneghini, Luigi F. ; Allende, Gloria ; Skyler, Jay S ; Alejandro, Rodolfo ; Pugliese, Alberto ; Kenyon, Norma S. / Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes. In: Clinical Immunology. 2011 ; Vol. 139, No. 3. pp. 290-301.
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