Immune hyperactivation of HIV-1-infected T cells mediated by Tat and the CD28 pathway

Melanie Ott, Stephane Emiliani, Carine Van Lint, Georges Herbein, Jennie Lovett, Narendra Chirmule, Thomas McCloskey, Savita G Pahwa, Eric Verdin

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

Human immunodeficiency virus-type 1 (HIV-1) infection is characterized by a chronic state of immune hyperactivation in patients. Infection of human peripheral blood lymphocytes with HIV-1 in vitro resulted in increased in terleukin-2 (IL-2) secretion in response to T cell activation via the CD3 and CD28 receptors. Expression of the HIV-1 transactivator Tat recapitulated this phenotype and was associated with increased IL-2 secretion in response to costimulation with CD3 plus CD28. IL-2 superinduction by Tat occurred at the transcriptional level, was mediated by the CD28-responsive element in the IL- 2 promoter, and was exclusively dependent on the 29 amino acids encoded by the second exon of Tat.

Original languageEnglish
Pages (from-to)1481-1485
Number of pages5
JournalScience
Volume275
Issue number5305
DOIs
StatePublished - Mar 7 1997
Externally publishedYes

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HIV-1
T-Lymphocytes
Trans-Activators
Virus Diseases
Interleukin-2
Exons
Lymphocytes
Phenotype
Amino Acids
Infection
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Ott, M., Emiliani, S., Van Lint, C., Herbein, G., Lovett, J., Chirmule, N., ... Verdin, E. (1997). Immune hyperactivation of HIV-1-infected T cells mediated by Tat and the CD28 pathway. Science, 275(5305), 1481-1485. https://doi.org/10.1126/science.275.5305.1481

Immune hyperactivation of HIV-1-infected T cells mediated by Tat and the CD28 pathway. / Ott, Melanie; Emiliani, Stephane; Van Lint, Carine; Herbein, Georges; Lovett, Jennie; Chirmule, Narendra; McCloskey, Thomas; Pahwa, Savita G; Verdin, Eric.

In: Science, Vol. 275, No. 5305, 07.03.1997, p. 1481-1485.

Research output: Contribution to journalArticle

Ott, M, Emiliani, S, Van Lint, C, Herbein, G, Lovett, J, Chirmule, N, McCloskey, T, Pahwa, SG & Verdin, E 1997, 'Immune hyperactivation of HIV-1-infected T cells mediated by Tat and the CD28 pathway', Science, vol. 275, no. 5305, pp. 1481-1485. https://doi.org/10.1126/science.275.5305.1481
Ott M, Emiliani S, Van Lint C, Herbein G, Lovett J, Chirmule N et al. Immune hyperactivation of HIV-1-infected T cells mediated by Tat and the CD28 pathway. Science. 1997 Mar 7;275(5305):1481-1485. https://doi.org/10.1126/science.275.5305.1481
Ott, Melanie ; Emiliani, Stephane ; Van Lint, Carine ; Herbein, Georges ; Lovett, Jennie ; Chirmule, Narendra ; McCloskey, Thomas ; Pahwa, Savita G ; Verdin, Eric. / Immune hyperactivation of HIV-1-infected T cells mediated by Tat and the CD28 pathway. In: Science. 1997 ; Vol. 275, No. 5305. pp. 1481-1485.
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