Immortalization of human T lymphocytes after transfection of Epstein-Barr virus DNA

Mario Stevenson, B. Volsky, M. Hedenskog, D. J. Volsky

Research output: Contribution to journalArticle

Abstract

Epstein-Barr virus (EBV), a ubiquitous human herpesvirus, has the ability to transform human B lymphocytes. No other cell type has been experimentally transformed by EBV, either by intact virions or naked viral DNA and subgenomic fragments. Two immortalized human T-lymphoblastoid cell lines have now been established by transfecting cord blood lymphocytes with purified B95-8 viral DNA enclosed in fusogenic Sendai virus envelopes (RSVE) and then exposing the cells to EBV from a P3HR-1 cell subclone. One of these lines, which has been fully characterized, is termed HBD-1. This line is positive for EBV DNA and expresses surface OKT11, OKT4, and Tac receptors, but not M-1, μ immunoglobulin chains, EBV receptors, or B-1 surface markers. The cells contain fully rearranged T-cell receptor genes and germline immunoglobulin genes. The karyotype of the cells is normal, they do not require interleukin-2 for growth, and do not contain human T-lymphotropic virus type I. However, the HBD-1 cells contain incomplete EBV genomes and express several EBV-determined antigens, including the early antigen type D, membrane antigens, but not EBV-determined nuclear antigen (EBNA). This association of the EBV genome with permanently growing hematopoietic cells of non B-cells lineage should prove useful in studies on the mechanism of EBV-mediated cell transformation.

Original languageEnglish
Pages (from-to)980-984
Number of pages5
JournalScience
Volume233
Issue number4767
StatePublished - Jan 1 1986
Externally publishedYes

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Human Herpesvirus 4
Transfection
T-Lymphocytes
DNA
Viral DNA
Antigens
B-Lymphocytes
Immunoglobulin Subunits
Complement 3d Receptors
Genome
Epstein-Barr Virus Nuclear Antigens
Defective Viruses
T-Cell Receptor Genes
Sendai virus
Immunoglobulin Genes
Herpesviridae
Cell Lineage
Fetal Blood
Karyotype
Virion

ASJC Scopus subject areas

  • General

Cite this

Stevenson, M., Volsky, B., Hedenskog, M., & Volsky, D. J. (1986). Immortalization of human T lymphocytes after transfection of Epstein-Barr virus DNA. Science, 233(4767), 980-984.

Immortalization of human T lymphocytes after transfection of Epstein-Barr virus DNA. / Stevenson, Mario; Volsky, B.; Hedenskog, M.; Volsky, D. J.

In: Science, Vol. 233, No. 4767, 01.01.1986, p. 980-984.

Research output: Contribution to journalArticle

Stevenson, M, Volsky, B, Hedenskog, M & Volsky, DJ 1986, 'Immortalization of human T lymphocytes after transfection of Epstein-Barr virus DNA', Science, vol. 233, no. 4767, pp. 980-984.
Stevenson M, Volsky B, Hedenskog M, Volsky DJ. Immortalization of human T lymphocytes after transfection of Epstein-Barr virus DNA. Science. 1986 Jan 1;233(4767):980-984.
Stevenson, Mario ; Volsky, B. ; Hedenskog, M. ; Volsky, D. J. / Immortalization of human T lymphocytes after transfection of Epstein-Barr virus DNA. In: Science. 1986 ; Vol. 233, No. 4767. pp. 980-984.
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