Immortalization in culture: Occurrence at a late stage in the progression of breast cancer

Helene S. Smith, Sandra R. Wolman, Shahnaz H. Dairkee, Miriam C. Hancock, Marc Lippman, Alena Leff, Adeline J. Hackett

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


The properties in culture of 3 breast cancer effusion metastases, obtained over approximately 2 years from the same patient, were examined. Despite repeated attempts with cryopreserved cells, only the last specimen reproducibly exhibited immortality in culture; the first 2 specimens grew initially but failed to develop into cell lines. Each specimen was unique in morphology and growth properties, although karyotypic markers indicated a common origin. Aberrations of chromosomes 1 and 11 marked these near-diploid cells, and further structural alterations of chromosome 11 accompanied the transition of biological properties observed in the third specimen.

Original languageEnglish (US)
Pages (from-to)611-615
Number of pages5
JournalJournal of the National Cancer Institute
Issue number4
StatePublished - Apr 1987
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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