Immobilization of D-ribulose-1,5-bisphosphate carboxylase/oxygenase: A step toward carbon dioxide fixation bioprocess

Subhra Chakrabarti, Sumana Bhattacharya, Sanjoy K. Bhattacharya

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Immobilization of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from spinach leaves is described. This enzyme enables the fixation of carbon dioxide on a five-carbon sugar D-ribulose-1,5-bisphosphate (RuBP). Two different immobilization methods were employed: dicyclohexylcarbodiimide coupling on nylon membrane matrix and dimethylpimelimidate immobilization on protein A agarose. The reusability of immobilized enzymes, coupling efficiency, and temperature-activity relationship of soluble and immobilized Rubisco are presented. The immobilization imparted greater thermal and storage stability. The thermal deactivation rates of the immobilized enzymes were considerably lower than those of the soluble enzyme.

Original languageEnglish (US)
Pages (from-to)705-711
Number of pages7
JournalBiotechnology and Bioengineering
Volume81
Issue number6
DOIs
StatePublished - Mar 20 2003
Externally publishedYes

Keywords

  • Bioprocess
  • Carbon dioxide fixation
  • Immobilization
  • Rubisco

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

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