Imaging dynamic molecular signaling by the Cdc42 GTPase within the developing CNS

Nima Sharifai, Hasitha Samarajeewa, Daichi Kamiyama, Tzyy Chyn Deng, Maria Boulina, Akira Chiba

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Protein interactions underlie the complexity of neuronal function. Potential interactions between specific proteins in the brain are predicted from assays based on genetic interaction and/or biochemistry. Genetic interaction reveals endogenous, but not necessarily direct, interactions between the proteins. Biochemistry-based assays, on the other hand, demonstrate direct interactions between proteins, but often outside their native environment or without a subcellular context. We aimed to achieve the best of both approaches by visualizing protein interaction directly within the brain of a live animal. Here, we show a proof-of-principle experiment in which the Cdc42 GTPase associates with its alleged partner WASp within neurons during the time and space that coincide with the newly developing CNS.

Original languageEnglish (US)
Article numbere88870
JournalPloS one
Volume9
Issue number2
DOIs
StatePublished - Feb 19 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Sharifai, N., Samarajeewa, H., Kamiyama, D., Deng, T. C., Boulina, M., & Chiba, A. (2014). Imaging dynamic molecular signaling by the Cdc42 GTPase within the developing CNS. PloS one, 9(2), [e88870]. https://doi.org/10.1371/journal.pone.0088870