Imaging cortical lesions in multiple sclerosis with ultra-high-field magnetic resonance imaging

David Pitt, Aaron Boster, Wei Pei, Eric Wohleb, Adam Jasne, Cherian R. Zachariah, Kottil W Rammohan, Michael V. Knopp, Petra Schmalbrock

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Objective: To determine the sensitivity of T2*-weighted gradient-echo (T2*GRE) and inversion recovery turbo-field-echo (TFE) sequences for cortical multiple sclerosis lesions at 7 T. Design, Setting, and Participants: Autopsied brain tissue from individuals with multiple sclerosis was scanned with 3-dimensional T2*GRE and 3-dimensional inversion recovery white matter-attenuated TFE sequences at 7 T. Cortical lesions visible with either sequence were scored for each anatomical lesion type. Imaged brain tissue was then processed for immunohistochemical analysis, and cortical lesions were identified by labeling with antibody against myelin basic protein and CD68 for microglia. Magnetic resonance images were matched with corresponding histological sections and scored retrospectively to determine the sensitivity for each cortical lesion type. Main Outcome Measure: Cortical lesion detection by 3-dimensional T2*GRE and white matter-attenuated TFE sequences. Results: The 3-dimensional T2*GRE and white matter-attenuated TFE sequences retrospectively detected 93% and 82% of all cortical lesions, respectively (with varying sensitivities for different lesion types). Lesion visibility was primarily determined by size as all undetected lesions were smaller than 1.1 mm at their smallest diameter. The T2*GRE images showed hypointense rings in some cortical lesions that corresponded with increased density of activated microglia. Conclusions: Three-dimensional T2*GREand white matter-attenuated TFE sequences at a 7-T field strength detect most cortical lesions in postmortem multiple sclerosis tissue. This study indicates the potential of T2*GRE and white matter-attenuated TFE sequences in ultra-high-field magnetic resonance imaging for cortical lesion detection in patients with multiple sclerosis.

Original languageEnglish
Pages (from-to)812-818
Number of pages7
JournalArchives of Neurology
Volume67
Issue number7
DOIs
StatePublished - Jul 1 2010
Externally publishedYes

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Multiple Sclerosis
Magnetic Resonance Imaging
Microglia
Myelin Basic Protein
Brain
Magnetic Resonance Spectroscopy
Outcome Assessment (Health Care)
White Matter
Imaging
Lesion
Antibodies

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Pitt, D., Boster, A., Pei, W., Wohleb, E., Jasne, A., Zachariah, C. R., ... Schmalbrock, P. (2010). Imaging cortical lesions in multiple sclerosis with ultra-high-field magnetic resonance imaging. Archives of Neurology, 67(7), 812-818. https://doi.org/10.1001/archneurol.2010.148

Imaging cortical lesions in multiple sclerosis with ultra-high-field magnetic resonance imaging. / Pitt, David; Boster, Aaron; Pei, Wei; Wohleb, Eric; Jasne, Adam; Zachariah, Cherian R.; Rammohan, Kottil W; Knopp, Michael V.; Schmalbrock, Petra.

In: Archives of Neurology, Vol. 67, No. 7, 01.07.2010, p. 812-818.

Research output: Contribution to journalArticle

Pitt, D, Boster, A, Pei, W, Wohleb, E, Jasne, A, Zachariah, CR, Rammohan, KW, Knopp, MV & Schmalbrock, P 2010, 'Imaging cortical lesions in multiple sclerosis with ultra-high-field magnetic resonance imaging', Archives of Neurology, vol. 67, no. 7, pp. 812-818. https://doi.org/10.1001/archneurol.2010.148
Pitt, David ; Boster, Aaron ; Pei, Wei ; Wohleb, Eric ; Jasne, Adam ; Zachariah, Cherian R. ; Rammohan, Kottil W ; Knopp, Michael V. ; Schmalbrock, Petra. / Imaging cortical lesions in multiple sclerosis with ultra-high-field magnetic resonance imaging. In: Archives of Neurology. 2010 ; Vol. 67, No. 7. pp. 812-818.
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abstract = "Objective: To determine the sensitivity of T2*-weighted gradient-echo (T2*GRE) and inversion recovery turbo-field-echo (TFE) sequences for cortical multiple sclerosis lesions at 7 T. Design, Setting, and Participants: Autopsied brain tissue from individuals with multiple sclerosis was scanned with 3-dimensional T2*GRE and 3-dimensional inversion recovery white matter-attenuated TFE sequences at 7 T. Cortical lesions visible with either sequence were scored for each anatomical lesion type. Imaged brain tissue was then processed for immunohistochemical analysis, and cortical lesions were identified by labeling with antibody against myelin basic protein and CD68 for microglia. Magnetic resonance images were matched with corresponding histological sections and scored retrospectively to determine the sensitivity for each cortical lesion type. Main Outcome Measure: Cortical lesion detection by 3-dimensional T2*GRE and white matter-attenuated TFE sequences. Results: The 3-dimensional T2*GRE and white matter-attenuated TFE sequences retrospectively detected 93{\%} and 82{\%} of all cortical lesions, respectively (with varying sensitivities for different lesion types). Lesion visibility was primarily determined by size as all undetected lesions were smaller than 1.1 mm at their smallest diameter. The T2*GRE images showed hypointense rings in some cortical lesions that corresponded with increased density of activated microglia. Conclusions: Three-dimensional T2*GREand white matter-attenuated TFE sequences at a 7-T field strength detect most cortical lesions in postmortem multiple sclerosis tissue. This study indicates the potential of T2*GRE and white matter-attenuated TFE sequences in ultra-high-field magnetic resonance imaging for cortical lesion detection in patients with multiple sclerosis.",
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AU - Rammohan, Kottil W

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N2 - Objective: To determine the sensitivity of T2*-weighted gradient-echo (T2*GRE) and inversion recovery turbo-field-echo (TFE) sequences for cortical multiple sclerosis lesions at 7 T. Design, Setting, and Participants: Autopsied brain tissue from individuals with multiple sclerosis was scanned with 3-dimensional T2*GRE and 3-dimensional inversion recovery white matter-attenuated TFE sequences at 7 T. Cortical lesions visible with either sequence were scored for each anatomical lesion type. Imaged brain tissue was then processed for immunohistochemical analysis, and cortical lesions were identified by labeling with antibody against myelin basic protein and CD68 for microglia. Magnetic resonance images were matched with corresponding histological sections and scored retrospectively to determine the sensitivity for each cortical lesion type. Main Outcome Measure: Cortical lesion detection by 3-dimensional T2*GRE and white matter-attenuated TFE sequences. Results: The 3-dimensional T2*GRE and white matter-attenuated TFE sequences retrospectively detected 93% and 82% of all cortical lesions, respectively (with varying sensitivities for different lesion types). Lesion visibility was primarily determined by size as all undetected lesions were smaller than 1.1 mm at their smallest diameter. The T2*GRE images showed hypointense rings in some cortical lesions that corresponded with increased density of activated microglia. Conclusions: Three-dimensional T2*GREand white matter-attenuated TFE sequences at a 7-T field strength detect most cortical lesions in postmortem multiple sclerosis tissue. This study indicates the potential of T2*GRE and white matter-attenuated TFE sequences in ultra-high-field magnetic resonance imaging for cortical lesion detection in patients with multiple sclerosis.

AB - Objective: To determine the sensitivity of T2*-weighted gradient-echo (T2*GRE) and inversion recovery turbo-field-echo (TFE) sequences for cortical multiple sclerosis lesions at 7 T. Design, Setting, and Participants: Autopsied brain tissue from individuals with multiple sclerosis was scanned with 3-dimensional T2*GRE and 3-dimensional inversion recovery white matter-attenuated TFE sequences at 7 T. Cortical lesions visible with either sequence were scored for each anatomical lesion type. Imaged brain tissue was then processed for immunohistochemical analysis, and cortical lesions were identified by labeling with antibody against myelin basic protein and CD68 for microglia. Magnetic resonance images were matched with corresponding histological sections and scored retrospectively to determine the sensitivity for each cortical lesion type. Main Outcome Measure: Cortical lesion detection by 3-dimensional T2*GRE and white matter-attenuated TFE sequences. Results: The 3-dimensional T2*GRE and white matter-attenuated TFE sequences retrospectively detected 93% and 82% of all cortical lesions, respectively (with varying sensitivities for different lesion types). Lesion visibility was primarily determined by size as all undetected lesions were smaller than 1.1 mm at their smallest diameter. The T2*GRE images showed hypointense rings in some cortical lesions that corresponded with increased density of activated microglia. Conclusions: Three-dimensional T2*GREand white matter-attenuated TFE sequences at a 7-T field strength detect most cortical lesions in postmortem multiple sclerosis tissue. This study indicates the potential of T2*GRE and white matter-attenuated TFE sequences in ultra-high-field magnetic resonance imaging for cortical lesion detection in patients with multiple sclerosis.

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