IL-8 induces the locomotion of human IL-2-activated natural killer cells: Involvement of a guanine nucleotide binding (G(o)) protein

K. Sebok, D. Woodside, A. Al-Aoukaty, A. D. Ho, S. Gluck, A. A. Maghazachi

Research output: Contribution to journalArticle

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Abstract

The effect of IL-8 on the in vitro locomotion of human IL-2-activated natural killer (IANK) cells was studied. It was observed that IL-8 induces chemokinesis in these cells, as determined by their migration in modified Boyden chambers. Bacterial toxins such as cholera toxin or pertussis toxin inhibited IL-8-induced chemokinetic activity, suggesting the involvement of guanine nucleotide-binding (G) proteins in IL-8 signal transduction in these cells. Pertussis toxin ADP-ribosylates a 39-kDa protein, whereas cholera toxin ADP-ribosylates a 43- to 45-kDa protein. Pretreatment of IANK cell membranes with 0.01 or 0.1 ng/ml of IL-8 and/or 5 μM GTP-γS did not affect pertussis toxin- or cholera toxin-dependent ADP-ribosylation. Western blot analysis showed that IANK cell membranes possess one G(i) (39 kDa), two G(s) (43 kDa and 45 kDa), and one G(o) (39 kDa). Pretreatment of IANK cell membranes with concentrations between 0.001 to 1.0 ng/ml of IL-8 resulted in the disappearance of the 39 kDa G(o), but not G(i) or G(s) protein(s), suggesting that IL-8 receptors expressed on IANK cells are coupled to G(o). Various concentrations of IL-8 enhanced the binding of GTP-γ35S to IANK cell membranes, which further indicates the coupling of G proteins to IL-8 receptors in IANK cells.

Original languageEnglish
Pages (from-to)1524-1534
Number of pages11
JournalJournal of Immunology
Volume150
Issue number4
StatePublished - Jan 1 1993
Externally publishedYes

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Gi-Go GTP-Binding Protein alpha Subunits
Guanine Nucleotides
Locomotion
Interleukin-8
Natural Killer Cells
Interleukin-2
Cholera Toxin
Pertussis Toxin
Interleukin-8 Receptors
Cell Membrane
Adenosine Diphosphate
Guanosine Triphosphate
GTP-Binding Proteins
Bacterial Toxins
Proteins
Interleukin-5
Signal Transduction
Carrier Proteins
Western Blotting

ASJC Scopus subject areas

  • Immunology

Cite this

Sebok, K., Woodside, D., Al-Aoukaty, A., Ho, A. D., Gluck, S., & Maghazachi, A. A. (1993). IL-8 induces the locomotion of human IL-2-activated natural killer cells: Involvement of a guanine nucleotide binding (G(o)) protein. Journal of Immunology, 150(4), 1524-1534.

IL-8 induces the locomotion of human IL-2-activated natural killer cells : Involvement of a guanine nucleotide binding (G(o)) protein. / Sebok, K.; Woodside, D.; Al-Aoukaty, A.; Ho, A. D.; Gluck, S.; Maghazachi, A. A.

In: Journal of Immunology, Vol. 150, No. 4, 01.01.1993, p. 1524-1534.

Research output: Contribution to journalArticle

Sebok, K, Woodside, D, Al-Aoukaty, A, Ho, AD, Gluck, S & Maghazachi, AA 1993, 'IL-8 induces the locomotion of human IL-2-activated natural killer cells: Involvement of a guanine nucleotide binding (G(o)) protein', Journal of Immunology, vol. 150, no. 4, pp. 1524-1534.
Sebok, K. ; Woodside, D. ; Al-Aoukaty, A. ; Ho, A. D. ; Gluck, S. ; Maghazachi, A. A. / IL-8 induces the locomotion of human IL-2-activated natural killer cells : Involvement of a guanine nucleotide binding (G(o)) protein. In: Journal of Immunology. 1993 ; Vol. 150, No. 4. pp. 1524-1534.
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