IL-4-induced oxidative stress upregulates VCAM-1 gene expression in human endothelial cells

Yong Woo Lee, Hartmut Kühn, Bernhard Hennig, Andrew S. Neish, Michal J Toborek

Research output: Contribution to journalArticle

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Abstract

Vascular cell adhesion molecule-1 (VCAM-1) is expressed in early stages of atherosclerosis: however, the mechanisms of its upregulation are not fully understood. In the present study, we examined the effects of interleukin-4 (IL-4) on VCAM-1 gene expression and its transcriptional regulatory mechanism in human umbilical vein endothelial cells (HUVEC). Reverse transcription-polymerase chain reaction showed that VCAM-1 mRNA was induced in IL-4-treated HUVEC in a time- and close-dependent manner. Among known transcription factors that have binding sites in the promoter region of the VCAM-1 gene, IL-4 activated only SP-1. In contrast, nuclear factor-κB (NF-κB), activator protein-1 (AP-1) and interferon regulatory factor-1 (IRF-1), which also have consensus binding sequences in the 5′-flanking region of the human VCAM-1 gene, were not activated. The role of SP-1 in IL-4-induced VCAM-1 expression was confirmed in HUVEC transfected with a reporter construct of the VCAM-1 promoter with mutated SP-1 binding site. As IL-4 treatment of HUVEC enhanced the intracellular oxidizing potential, as indicated by an increase in 2′,7′-dichlorofluorescein (DCF) fluorescence, we studied the effect of antioxidants on IL-4-induced VCAM-1 expression. Pretreatment of HUVEC with pyrrolidine dithiocarbamate (PDTC) or N-acetylcysteine (NAC) completely prevented IL-4-induced VCAM-1 expression. In addition, PDTC inhibited IL-4-related activation of SP-1. These results suggest that IL-4-induced oxidative stress upregulates the expression of VCAM-1 gene in HUVEC at transcriptional levels via activation of SP-1 transcription factor. In contrast, NF-κB, AP-1 or IRF-1 do not appear to be involved in the signal transduction cascade.

Original languageEnglish
Pages (from-to)83-94
Number of pages12
JournalJournal of Molecular and Cellular Cardiology
Volume33
Issue number1
DOIs
StatePublished - Feb 19 2001
Externally publishedYes

Fingerprint

Vascular Cell Adhesion Molecule-1
Interleukin-4
Oxidative Stress
Up-Regulation
Endothelial Cells
Human Umbilical Vein Endothelial Cells
Gene Expression
Interferon Regulatory Factor-1
Transcription Factor AP-1
Transcription Factors
Binding Sites
Genes
5' Flanking Region
Consensus Sequence
Acetylcysteine
Genetic Promoter Regions
Reverse Transcription
Signal Transduction
Atherosclerosis
Antioxidants

Keywords

  • Atherosclerosis
  • Human endothelial cells
  • Interleukin-4
  • Oxidative stress
  • Transcriptional regulation
  • Vascular cell adhesion molecule-1

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

IL-4-induced oxidative stress upregulates VCAM-1 gene expression in human endothelial cells. / Lee, Yong Woo; Kühn, Hartmut; Hennig, Bernhard; Neish, Andrew S.; Toborek, Michal J.

In: Journal of Molecular and Cellular Cardiology, Vol. 33, No. 1, 19.02.2001, p. 83-94.

Research output: Contribution to journalArticle

Lee, Yong Woo ; Kühn, Hartmut ; Hennig, Bernhard ; Neish, Andrew S. ; Toborek, Michal J. / IL-4-induced oxidative stress upregulates VCAM-1 gene expression in human endothelial cells. In: Journal of Molecular and Cellular Cardiology. 2001 ; Vol. 33, No. 1. pp. 83-94.
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AB - Vascular cell adhesion molecule-1 (VCAM-1) is expressed in early stages of atherosclerosis: however, the mechanisms of its upregulation are not fully understood. In the present study, we examined the effects of interleukin-4 (IL-4) on VCAM-1 gene expression and its transcriptional regulatory mechanism in human umbilical vein endothelial cells (HUVEC). Reverse transcription-polymerase chain reaction showed that VCAM-1 mRNA was induced in IL-4-treated HUVEC in a time- and close-dependent manner. Among known transcription factors that have binding sites in the promoter region of the VCAM-1 gene, IL-4 activated only SP-1. In contrast, nuclear factor-κB (NF-κB), activator protein-1 (AP-1) and interferon regulatory factor-1 (IRF-1), which also have consensus binding sequences in the 5′-flanking region of the human VCAM-1 gene, were not activated. The role of SP-1 in IL-4-induced VCAM-1 expression was confirmed in HUVEC transfected with a reporter construct of the VCAM-1 promoter with mutated SP-1 binding site. As IL-4 treatment of HUVEC enhanced the intracellular oxidizing potential, as indicated by an increase in 2′,7′-dichlorofluorescein (DCF) fluorescence, we studied the effect of antioxidants on IL-4-induced VCAM-1 expression. Pretreatment of HUVEC with pyrrolidine dithiocarbamate (PDTC) or N-acetylcysteine (NAC) completely prevented IL-4-induced VCAM-1 expression. In addition, PDTC inhibited IL-4-related activation of SP-1. These results suggest that IL-4-induced oxidative stress upregulates the expression of VCAM-1 gene in HUVEC at transcriptional levels via activation of SP-1 transcription factor. In contrast, NF-κB, AP-1 or IRF-1 do not appear to be involved in the signal transduction cascade.

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