IL-2R signaling is essential for functional maturation of regulatory T cells during thymic development

Guoyan Cheng, Aixin Yu, Michael J. Dee, Thomas Malek

Research output: Contribution to journalArticle

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Abstract

CD4+ Foxp3+ regulatory T cells (Tregs) are an independent cell lineage, and their developmental progression during thymic development depends on IL-2R signaling. However, the role of IL-2R signaling during thymic Treg development remains only partially understood. The current study assessed the contribution of IL-2 to the expansion and functional programming of developing Tregs. In the absence of IL-2Rβ signaling, predominantly CD4+ CD25- Foxp3lo T cells were found, and these cells exhibited somewhat lower expression of the proliferative marker Ki67. These immature Tregs, which represent products of failed development, were also found in normal mice and were characterized by markedly lower expression of several Treg functional molecules. Therefore, IL-2R is required for the progression, functional programming, and expansion of Tregs during thymic development. An IL-2R-signaling mutant that lowers STAT5 activation readily supported Treg functional programming, but Treg proliferation remained somewhat impaired. The requirement for IL-2 during thymic Treg expansion was best illustrated in mixed chimeras where the Tregs with mutant IL-2Rs were forced to compete with wild-type Tregs during their development. Tregs with impaired IL-2R signaling were more prevalent in the thymus than spleen in these competitive experiments. The general effectiveness of mutant IL-2Rs to support thymic Treg development is partially accounted for by a heightened capacity of thymic Tregs to respond to IL-2. Overall, our data support a model in which limiting IL-2R signaling is amplified by thymic Tregs to readily support their development and functional programming, whereas these same conditions are not sufficient to support peripheral Treg homeostasis.

Original languageEnglish
Pages (from-to)1567-1575
Number of pages9
JournalJournal of Immunology
Volume190
Issue number4
DOIs
StatePublished - Feb 15 2013

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Regulatory T-Lymphocytes
Interleukin-2
Cell Lineage
Thymus Gland
Homeostasis
Spleen
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

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IL-2R signaling is essential for functional maturation of regulatory T cells during thymic development. / Cheng, Guoyan; Yu, Aixin; Dee, Michael J.; Malek, Thomas.

In: Journal of Immunology, Vol. 190, No. 4, 15.02.2013, p. 1567-1575.

Research output: Contribution to journalArticle

Cheng, Guoyan ; Yu, Aixin ; Dee, Michael J. ; Malek, Thomas. / IL-2R signaling is essential for functional maturation of regulatory T cells during thymic development. In: Journal of Immunology. 2013 ; Vol. 190, No. 4. pp. 1567-1575.
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